APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
Application |
Recommended Dilution |
1:500-1:3000 |
1:100-1:1000 |
1:100-1:1000 |
Not tested in other applications.
Calculated MW
Positive Control
A549 , HeLa
Predict Reactivity
Mouse, Rat, Rabbit, Bovine(>80% identity)
PROPERTIES
Form
Liquid
Buffer
0.1M Tris, 0.1M Glycine, 20% Glycerol
Preservative
0.01% Thimerosal
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Recombinant protein encompassing a sequence within the center region of human PATZ1. The exact sequence is proprietary.
Purification
Purified by antigen-affinity chromatography.
Conjugation
Unconjugated
RRID
AB_10622178
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
POZ/BTB and AT hook containing zinc finger 1 , MAZR , PATZ , RIAZ , ZBTB19 , ZNF278 , ZSG , dJ400N23
Cellular Localization
Nucleus
Background
The protein encoded by this gene contains an A-T hook DNA binding motif which usually binds to other DNA binding structures to play an important role in chromatin modeling and transcription regulation. Its Poz domain is thought to function as a site for protein-protein interaction and is required for transcriptional repression, and the zinc-fingers comprise the DNA binding domain. Since the encoded protein has typical features of a transcription factor, it is postulated to be a repressor of gene expression. In small round cell sarcoma, this gene is fused to EWS by a small inversion of 22q, then the hybrid is thought to be translocated (t(1;22)(p36.1;q12). The rearrangement of chromosome 22 involves intron 8 of EWS and exon 1 of this gene creating a chimeric sequence containing the transactivation domain of EWS fused to zinc finger domain of this protein. This is a distinct example of an intra-chromosomal rearrangement of chromosome 22. Four alternatively spliced transcript variants are described for this gene. [provided by RefSeq]
Database
Research Area
DATA IMAGES
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GTX115725 ICC/IF Image
Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using PATZ1(GTX115725) antibody at 1:200 dilution.
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GTX115725 IHC-P Image
Immunohistochemical analysis of paraffin-embedded N87 xenograft, using PATZ1(GTX115725) antibody at 1:500 dilution.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GTX115725 WB Image
Sample (30 ug of whole cell lysate) A: A549 B: Hela 7.5% SDS PAGE GTX115725 diluted at 1:1000
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REFERENCE
There are currently no references for PATZ1 antibody [N3C2], Internal (GTX115725). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for PATZ1 antibody [N3C2], Internal (GTX115725). Be the first to share your experience with this product.