GeneTex
United States (US)

PATZ1 antibody [N3C2], Internal

GTX115725_40296_IFA_18121410_136.jpg
GTX115725_40296_IHC_18121410_684.jpg
GTX115725_WB_18121410_510.jpg

Cat No. GTX115725

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P
Reactivity Human
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

74 kDa. ( Note )

Positive Control

A549 , HeLa , HepG2 , HCT116

Predict Reactivity

Mouse, Rat, Rabbit, Bovine(>80% identity)
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human PATZ1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Poz/Btb And At Hook Containing Zinc Finger 1,Mazr,Patz,Riaz,Zbtb19,Znf278,Zsg,Dj400N23,Patz1

Cellular Localization

Nucleus

Background

The protein encoded by this gene contains an A-T hook DNA binding motif which usually binds to other DNA binding structures to play an important role in chromatin modeling and transcription regulation. Its Poz domain is thought to function as a site for protein-protein interaction and is required for transcriptional repression, and the zinc-fingers comprise the DNA binding domain. Since the encoded protein has typical features of a transcription factor, it is postulated to be a repressor of gene expression. In small round cell sarcoma, this gene is fused to EWS by a small inversion of 22q, then the hybrid is thought to be translocated (t(1;22)(p36.1;q12). The rearrangement of chromosome 22 involves intron 8 of EWS and exon 1 of this gene creating a chimeric sequence containing the transactivation domain of EWS fused to zinc finger domain of this protein. This is a distinct example of an intra-chromosomal rearrangement of chromosome 22. Four alternatively spliced transcript variants are described for this gene. [provided by RefSeq]

Database

Research Area

DATA IMAGES
GTX115725_40296_IFA_18121410_136.jpg

GTX115725 ICC/IF Image

Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using PATZ1(GTX115725) antibody at 1:200 dilution.

GTX115725_40296_IHC_18121410_684.jpg

GTX115725 IHC-P Image

Immunohistochemical analysis of paraffin-embedded N87 xenograft, using PATZ1(GTX115725) antibody at 1:500 dilution.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX115725_WB_18121410_510.jpg

GTX115725 WB Image

Sample (30 ug of whole cell lysate)
A: A549
B: Hela
7.5% SDS PAGE
GTX115725 diluted at 1:1000

SDS
Glycerol.pdf
Thimerosal.pdf