PATZ1 antibody

Cat. No. GTX04950

Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Application	WB, ICC/IF, FACS, ELISA
Reactivity	Human
Package	100 μg ($469)

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Datasheet

View product citations for antibody GTX04950 on CiteAb

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Recommended Dilution
WB	0.25-0.5 μg/ml
ICC/IF	5 μg/ml
FACS	1-3 μg/1x10⁶ cells
ELISA	0.1-0.5 μg/ml
Note : FACS Permeabilization is required for this application.

Not tested in other applications.

Calculated MW

74 kDa. ( Note )

Predict Reactivity

Mouse(>80% identity)

PROPERTIES

Form

Liquid

Buffer

4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na₂HPO₄

Preservative

No preservatives

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.5 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

E.coli-derived human PATZ1 recombinant protein (Position: M145-Q680).

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

MAZR,PATZ,PATZ1,POZ/BTB and AT hook containing zinc finger 1,RIAZ,ZBTB19,ZNF278,ZSG,dJ400N23

Cellular Localization

Nucleus

Background

The protein encoded by this gene contains an A-T hook DNA binding motif which usually binds to other DNA binding structures to play an important role in chromatin modeling and transcription regulation. Its Poz domain is thought to function as a site for protein-protein interaction and is required for transcriptional repression, and the zinc-fingers comprise the DNA binding domain. Since the encoded protein has typical features of a transcription factor, it is postulated to be a repressor of gene expression. In small round cell sarcoma, this gene is fused to EWS by a small inversion of 22q, then the hybrid is thought to be translocated (t(1;22)(p36.1;q12). The rearrangement of chromosome 22 involves intron 8 of EWS and exon 1 of this gene creating a chimeric sequence containing the transactivation domain of EWS fused to zinc finger domain of this protein. This is a distinct example of an intra-chromosomal rearrangement of chromosome 22. Four alternatively spliced transcript variants are described for this gene. [provided by RefSeq, Jul 2008]

Database

Gene ID: 23598 PATZ1
UniProt: Q9HBE1 PATZ1

Research Area

DATA IMAGES

GTX04950 ICC/IF Image

ICC/IF analysis of PFA-fixed HeLa cells using GTX04950 PATZ1 antibody.
Red : Primary antibody
Green : Beta tubulin
Antigen retrieval : IHC enzyme antigen retrieval reagent
Dilution : 5 μg/mL

GTX04950 WB Image

WB analysis of various samples using GTX04950 PATZ1 antibody.
Loading : 30μg
Dilution : 0.5 μg/mL

GTX04950 FACS Image

FACS analysis of 4% PFA-fixed HeLa cells using GTX04950 PATZ1 antibody.
Blue line : Primary antibody
Green line : Isotype control
Red line : Unlabelled sample
Dilution : 1 μg/1x10⁶ cells
Permeabilization: PBS with saponin and triton

REFERENCE

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REVIEW

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Package

List Price ($)

100 μg

$ 469

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