APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 0.3-1μg/ml |
| 4.5μg/ml |
|
Note :
IHC-P
Human Esophagus expression of PAX3 is detected in squamous epithelium.
|
Not tested in other applications.
Calculated MW
Positive Control
The peptide used to generate this antibody is available for purchase (GTX89743-PEP).
Product Note
This antibody is expected to recognize all 8 reported isoforms (NP_852122.1; NP_000429.2; NP_039230.1; NP_852123.1; NP_852124.1; NP_852126.1; NP_852125.1; NP_001120838.1).
Predict Reactivity
Rat, Bovine(>80% identity)
PROPERTIES
Form
Liquid
Buffer
TBS, 0.5% BSA
Preservative
0.02% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
0.50 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Peptide with sequence TTLAGAVPRMM-C, from the N Terminus of the protein sequence according to NP_852122.1; NP_000429.2; NP_039230.1; NP_852123.1; NP_852124.1; NP_852126.1; NP_852125.1; NP_001120838.1.
Purification
Purified by ammonium sulphate precipitation followed by antigen affinity chromatography
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
paired box 3 , CDHS , HUP2 , WS1 , WS3
Cellular Localization
Nucleus
Background
This gene is a member of the paired box (PAX) family of transcription factors. Members of the PAX family typically contain a paired box domain and a paired-type homeodomain. These genes play critical roles during fetal development. Mutations in paired box gene 3 are associated with Waardenburg syndrome, craniofacial-deafness-hand syndrome, and alveolar rhabdomyosarcoma. The translocation t(2;13)(q35;q14), which represents a fusion between PAX3 and the forkhead gene, is a frequent finding in alveolar rhabdomyosarcoma. Alternative splicing results in transcripts encoding isoforms with different C-termini. [provided by RefSeq, Jul 2008]
Database
Research Area
DATA IMAGES
|
GTX89743 WB Image
WB analysis of human duodenum lysate using GTX89743 PAX3 antibody, N-term.
Dilution : 0.3μg/ml
Loading : 35μg protein in RIPA buffer
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GTX89743 IHC-P Image
IHC-P analysis of human esophagus using GTX89743 PAX3 antibody, N-term.
Antigen retrieval : citrate buffer pH 6
Dilution : 4.5μg/ml
|
REFERENCE
There are currently no references for PAX3 antibody, N-term (GTX89743). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for PAX3 antibody, N-term (GTX89743). Be the first to share your experience with this product.
