FACS analysis of permeabilized Ramos cells (red histogram) or PBS alone control(black histogram) using GTX54532 PAX5 antibody.
Fixation : 4% formaldehyde
Dilution : 1:50

ICC/IF analysis of Raji cells using GTX54532 PAX5 antibody. Cells were probed without (left) or with(right) an antibody.
Green : Primary antibody
Blue : Nuclei
Fixation : Formalin
Permeabilization : 0.1% Triton X-100 in TBS for 5-10 minute
Dilution : 1:100 and incubated overnight at 4ºC

IHC-P analysis of mouse lymph node tissue using GTX54532 PAX5 antibody.
Right : Primary antibody
Left : Negative control without primary antibody
Antigen retrieval : 10mM sodium citrate (pH 6.0), microwaved for 8-15 min
Dilution : 1:100

ICC/IF analysis of Ramos and negative control Jurkat cells using GTX54532 PAX5 antibody.
Green : Primary antibody
Blue : Nuclei
Red : Actin
Fixation : Formalin
Permeabilization : 0.1% Triton X-100 in TBS for 10 minutes
Dilution : 1:100 for at least 1 hour at room temperature

ICC/IF analysis of Ramos cells using GTX54532 PAX5 antibody. Cells were probed without (left) or with(right) an antibody.
Green : Primary antibody
Blue : Nuclei
Fixation : Formalin
Permeabilization : 0.1% Triton X-100 in TBS for 5-10 minute
Dilution : 1:100 and incubated overnight at 4ºC

IP analysis of Raji cell lysates using GTX54532 PAX5 antibody.
IP reaction : 2μg antibody / 750μg lysate
Dilution : 1:1000

WB analysis of 75μg of whole cell lysates from PAX5-expressing B cell lymphoma-derived cell lines, negative control Jurkat cells using GTX54532 PAX5 antibody.
Dilution : 1:1000

IHC-P analysis of human tonsil tissue using GTX54532 PAX5 antibody.
Right : Primary antibody
Left : Negative control without primary antibody
Antigen retrieval : 10mM sodium citrate (pH 6.0), microwaved for 8-15 min
Dilution : 1:200

The data was published in the journal Cancers (Basel) in 2020.PMID: 32650570