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PAX5 antibody

Orthogonal
Anti-PAX5 antibody used in IHC (Paraffin sections) (IHC-P). GTX54532
Anti-PAX5 antibody used in Western Blot (WB). GTX54532
Anti-PAX5 antibody used in Immunoprecipitation (IP). GTX54532
Anti-PAX5 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX54532
Anti-PAX5 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX54532
Anti-PAX5 antibody used in IHC (Paraffin sections) (IHC-P). GTX54532
Anti-PAX5 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX54532
Anti-PAX5 antibody used in Flow cytometry (FACS). GTX54532
Anti-PAX5 antibody used in IHC (Paraffin sections) (IHC-P). GTX54532

Cat. No. GTX54532

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P, FACS, IP

Reactivity

 Human, Mouse
Package
100 μg ($399)
See all PAX5 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:2000
ICC/IF 1:20-1:200
IHC-P 1:50-1:500
FACS 1:50
IP 2-5 μg
Not tested in other applications.

Calculated MW

42 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 1mg/ml BSA, 30% Glycerol

Preservative

0.05% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Purified internal fragment of human recombinant PAX5 expressed in E. coli

Purification

Protein G purified

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

paired box 5 , ALL3 , BSAP

Cellular Localization

Nucleus

Background

This gene encodes a member of the paired box (PAX) family of transcription factors. The central feature of this gene family is a novel, highly conserved DNA-binding motif, known as the paired box. Paired box transcription factors are important regulators in early development, and alterations in the expression of their genes are thought to contribute to neoplastic transformation. This gene encodes the B-cell lineage specific activator protein that is expressed at early, but not late stages of B-cell differentiation. Its expression has also been detected in developing CNS and testis and so the encoded protein may also play a role in neural development and spermatogenesis. This gene is located at 9p13, which is involved in t(9;14)(p13;q32) translocations recurring in small lymphocytic lymphomas of the plasmacytoid subtype, and in derived large-cell lymphomas. This translocation brings the potent E-mu enhancer of the IgH gene into close proximity of the PAX5 promoter, suggesting that the deregulation of transcription of this gene contributes to the pathogenesis of these lymphomas. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jul 2013]

Database

Research Area

DATA IMAGES

Anti-PAX5 antibody used in IHC (Paraffin sections) (IHC-P). GTX54532

GTX54532 IHC-P Image

IHC-P analysis of human tonsil tissue using GTX54532 PAX5 antibody.
Right : Primary antibody
Left : Negative control without primary antibody
Antigen retrieval : 10mM sodium citrate (pH 6.0), microwaved for 8-15 min
Dilution : 1:200

Anti-PAX5 antibody used in Western Blot (WB). GTX54532

GTX54532 WB Image

WB analysis of 75μg of whole cell lysates from PAX5-expressing B cell lymphoma-derived cell lines, negative control Jurkat cells using GTX54532 PAX5 antibody.
Dilution : 1:1000

Anti-PAX5 antibody used in Immunoprecipitation (IP). GTX54532

GTX54532 IP Image

IP analysis of Raji cell lysates using GTX54532 PAX5 antibody.
IP reaction : 2μg antibody / 750μg lysate
Dilution : 1:1000

Anti-PAX5 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX54532

GTX54532 ICC/IF Image

ICC/IF analysis of Ramos cells using GTX54532 PAX5 antibody. Cells were probed without (left) or with(right) an antibody.
Green : Primary antibody
Blue : Nuclei
Fixation : Formalin
Permeabilization : 0.1% Triton X-100 in TBS for 5-10 minute
Dilution : 1:100 and incubated overnight at 4ºC

Anti-PAX5 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX54532

GTX54532 ICC/IF Image

ICC/IF analysis of Ramos and negative control Jurkat cells using GTX54532 PAX5 antibody.
Green : Primary antibody
Blue : Nuclei
Red : Actin
Fixation : Formalin
Permeabilization : 0.1% Triton X-100 in TBS for 10 minutes
Dilution : 1:100 for at least 1 hour at room temperature

Anti-PAX5 antibody used in IHC (Paraffin sections) (IHC-P). GTX54532

GTX54532 IHC-P Image

IHC-P analysis of mouse lymph node tissue using GTX54532 PAX5 antibody.
Right : Primary antibody
Left : Negative control without primary antibody
Antigen retrieval : 10mM sodium citrate (pH 6.0), microwaved for 8-15 min
Dilution : 1:100

Anti-PAX5 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX54532

GTX54532 ICC/IF Image

ICC/IF analysis of Raji cells using GTX54532 PAX5 antibody. Cells were probed without (left) or with(right) an antibody.
Green : Primary antibody
Blue : Nuclei
Fixation : Formalin
Permeabilization : 0.1% Triton X-100 in TBS for 5-10 minute
Dilution : 1:100 and incubated overnight at 4ºC

Anti-PAX5 antibody used in Flow cytometry (FACS). GTX54532

GTX54532 FACS Image

FACS analysis of permeabilized Ramos cells (red histogram) or PBS alone control(black histogram) using GTX54532 PAX5 antibody.
Fixation : 4% formaldehyde
Dilution : 1:50

Anti-PAX5 antibody used in IHC (Paraffin sections) (IHC-P). GTX54532

GTX54532 IHC-P Image

Orthogonal

The data was published in the journal Cancers (Basel) in 2020.PMID: 32650570

REFERENCE

Application Reference

Chan YL et al. Cancers (Basel) 2020; 12 (7) TIAM2S Mediates Serotonin Homeostasis and Provokes a Pro-Inflammatory Immune Microenvironment Permissive for Colorectal Tumorigenesis.
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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399