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PAX6 antibody [GT9412]

Orthogonal
Anti-PAX6 antibody [GT9412] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX634863
Anti-PAX6 antibody [GT9412] used in Western Blot (WB). GTX634863
Anti-PAX6 antibody [GT9412] used in IHC (Paraffin sections) (IHC-P). GTX634863
Anti-PAX6 antibody [GT9412] used in IHC (Paraffin sections) (IHC-P). GTX634863

Cat. No. GTX634863

Reference 2

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 GT9412

Isotype

 IgG1

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)
See all PAX6 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:1000
ICC/IF Assay dependent
IHC-P Assay dependent
Not tested in other applications.

Calculated MW

47 kDa. ( Note )

Predict Reactivity

Rabbit, Bovine, Dog, Chicken, Pig, Xenopus tropicalis, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

No Preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, we recommend adding 50% glycerol, then aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.42 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human PAX6. The exact sequence is proprietary.

Purification

Affinity purified by Protein G.

Conjugation

Unconjugated

RRID

AB_2888491

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

paired box 6 , AN , AN2 , ASGD5 , D11S812E , FVH1 , MGDA , WAGR

Cellular Localization

Nucleus

Background

This gene encodes a homeobox and paired domain-containing protein that binds DNA and functions as a regulator of transcription. Activity of this protein is key in the development of neural tissues, particularly the eye. This gene is regulated by multiple enhancers located up to hundreds of kilobases distant from this locus. Mutations in this gene or in the enhancer regions can cause ocular disorders such as aniridia and Peter's anomaly. Use of alternate promoters and alternative splicing result in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jul 2015]

Database

Research Area

DATA IMAGES

Anti-PAX6 antibody [GT9412] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX634863

GTX634863 ICC/IF Image

PAX6 antibody [GT9412] detects PAX6 protein at nucleus by immunofluorescent analysis.
Sample: 293T cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: PAX6 stained by PAX6 antibody [GT9412] (GTX634863) diluted at 1:500.
Red: beta Tubulin, a cytoskeleton marker, stained by beta Tubulin antibody (GTX101279) diluted at 1:1000.

Anti-PAX6 antibody [GT9412] used in Western Blot (WB). GTX634863

GTX634863 WB Image

Orthogonal

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with PAX6 antibody [GT9412] (GTX634863) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

Anti-PAX6 antibody [GT9412] used in IHC (Paraffin sections) (IHC-P). GTX634863

GTX634863 IHC-P Image

PAX6 antibody [GT9412] detects PAX6 protein at nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded mouse eye.
Green: PAX6 stained by PAX6 antibody [GT9412] (GTX634863) diluted at 1:500.
Red: beta Tubulin 3/ Tuj1, a cytoskeleton marker, stained by beta Tubulin 3/ Tuj1 antibody (GTX130245) diluted at 1:250.
Blue: Fluoroshield with DAPI (GTX30920).
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-PAX6 antibody [GT9412] used in IHC (Paraffin sections) (IHC-P). GTX634863

GTX634863 IHC-P Image

PAX6 antibody [GT9412] detects PAX6 protein at nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded rat cerebellum.
PAX6 stained by PAX6 antibody [GT9412] (GTX634863) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

REFERENCE

Application Reference

Y Zhang et al. Stem Cell Res 2023; : 103201 Human induced pluripotent stem cell line from fibroblasts (HEBHMUi015-A) and chondrogenic differentiation
Application : ICC/IF
Mingjing Zhang et al. Stem Cell Research 2023; Human Induced pluripotent stem cell (iPSC) line (HEBHMUi014-A) derived from a patient with Alzheimer's disease
Application : ICC/IF
Reactivity : Human
Submit a Reference

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SDS
PBS.pdf
Glycerol.pdf
Package List Price ($)
$ 399
$ 169