PAX6 antibody [HL2599] detects PAX6 protein at nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded rat cerebellum.
PAX6 stained by PAX6 antibody [HL2599] (GTX639051) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

PAX6 antibody [HL2599] detects PAX6 protein at nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded rat eye.
Green: PAX6 stained by PAX6 antibody [HL2599] (GTX639051) diluted at 1:250.
Red: beta Tubulin 3/ Tuj1, a neural marker, stained by beta Tubulin 3/ Tuj1 antibody [GT11710] (GTX631836) diluted at 1:.
Blue: Fluoroshield with DAPI (GTX30920).
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

PAX6 antibody [HL2599] detects PAX6 protein at nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded mouse cerebellum.
PAX6 stained by PAX6 antibody [HL2599] (GTX639051) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

PAX6 antibody [HL2599] detects PAX6 protein at nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded mouse eye.
Green: PAX6 stained by PAX6 antibody [HL2599] (GTX639051) diluted at 1:250.
Red: beta Tubulin 3/ Tuj1, a neural marker, stained by beta Tubulin 3/ Tuj1 antibody [GT11710] (GTX631836) diluted at 1:.
Blue: Fluoroshield with DAPI (GTX30920).
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with PAX6 antibody [HL2599] (GTX639051) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

Whole cell extract (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with PAX6 antibody [HL2599] (GTX639051) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

PAX6 antibody [HL2599] detects PAX6 protein at nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: PAX6 stained by PAX6 antibody [HL2599] (GTX639051) diluted at 1:500.
Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000.

Rat tissue extract (50 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with PAX6 antibody [HL2599] (GTX639051) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Non-transfected (–) and transfected (+) 293T whole cell extracts were separated by 10% SDS-PAGE, and the membrane was blotted with PAX6 antibody [HL2599] (GTX639051) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Various tissue extracts (50 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with PAX6 antibody [HL2599] (GTX639051) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.