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PAX6 antibody, Internal

Anti-PAX6 antibody, Internal used in Western Blot (WB). GTX89029

Cat. No. GTX89029

Host

 Goat

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB

Reactivity

 Mouse
Package
100 μg ($399)
See all PAX6 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 0.3-1μg/ml
Not tested in other applications.

Calculated MW

47 kDa. ( Note )

Positive Control

The peptide used to generate this antibody is available for purchase (GTX89029-PEP).

Product Note

This antibody is expected to recognise both reported isoforms (NP_000271.1 and NP_001595.2).

Predict Reactivity

Human, Rat(>80% identity)

PROPERTIES

Form

Liquid

Buffer

TBS, 0.5% BSA

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.50 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Peptide with sequence C-REEKLRNQRRQASN, from the internal region of the protein sequence according to NP_000271.1; NP_001595.2.

Purification

Purified by ammonium sulphate precipitation followed by antigen affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

paired box 6 , AN , AN2 , ASGD5 , D11S812E , FVH1 , MGDA , WAGR

Cellular Localization

Nucleus

Background

This gene encodes a homeobox and paired domain-containing protein that binds DNA and functions as a regulator of transcription. Activity of this protein is key in the development of neural tissues, particularly the eye. This gene is regulated by multiple enhancers located up to hundreds of kilobases distant from this locus. Mutations in this gene or in the enhancer regions can cause ocular disorders such as aniridia and Peter's anomaly. Use of alternate promoters and alternative splicing result in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jul 2015]

Database

Research Area

DATA IMAGES

Anti-PAX6 antibody, Internal used in Western Blot (WB). GTX89029

GTX89029 WB Image

WB analysis of mouse eye lysate using GTX89029 PAX6 antibody, Internal.
Dilution : 0.5μg/ml
Loading : 35μg protein in RIPA buffer

REFERENCE

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REVIEW

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SDS
Sodium Azide.pdf
Package List Price ($)
$ 399