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PAX6 antibody [SPM612]

Anti-PAX6 antibody [SPM612] used in IHC (Paraffin sections) (IHC-P). GTX34957

Cat. No. GTX34957

Host

Mouse

Clonality

Monoclonal

Clone Name

SPM612

Isotype

IgG1

Application

ICC/IF, IHC-P, FACS

Reactivity

Human
Package
100 μg ($529)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
ICC/IF 1-2μg/ml
IHC-P 1-2μg/ml for 30 minutes at RT
FACS 1-2μg/10⁶ cells

Note :

IHC-P
Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95ºC followed by cooling at RT for 20 minutes.

Not tested in other applications.

Calculated MW

47 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 0.05% BSA

Preservative

0.05% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.2 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant fragment (N-terminus; aa 1-300) of human PAX6 protein

Purification

Protein A/G purified

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

AN , AN2 , D11S812E , FVH1 , MGDA , PAX6 , WAGR , paired box 6

Cellular Localization

Nucleus

Background

This gene encodes paired box protein Pax-6, one of many human homologs of the Drosophila melanogaster gene prd. In addition to a conserved paired box domain, a hallmark feature of this gene family, the encoded protein also contains a homeobox domain. Both domains are known to bind DNA and function as regulators of gene transcription. Activity of this protein is key in the development of neural tissues, particularly the eye. This gene is regulated by multiple enhancers located up to hundreds of kilobases distant from this locus. Mutations in this gene or in the enhancer regions can cause ocular disorders such as aniridia and Peter's anomaly. Use of alternate promoters and alternative splicing results in multiple transcript variants encoding different isoforms. Interestingly, inclusion of a particular alternate coding exon has been shown to increase the length of the paired box domain and alter its DNA binding specificity. Consequently, isoforms that carry the shorter paired box domain regulate a different set of genes compared to the isoforms carrying the longer paired box domain. [provided by RefSeq, Mar 2019]

Database

Research Area

DATA IMAGES

Anti-PAX6 antibody [SPM612] used in IHC (Paraffin sections) (IHC-P). GTX34957

GTX34957 IHC-P Image

IHC-P analysis of human gastric carcinoma tissue using GTX34957 PAX6 antibody [SPM612].

REFERENCE

There are currently no references for PAX6 antibody [SPM612] (GTX34957). Be the first to share your publications with this product.

REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 529