APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1:500-1:3000 |
| 1:100-1:1000 |
| 1:100-1:1000 |
| 1:100-1:1000 |
| 1:1000-1:1000 |
Not tested in other applications.
Calculated MW
Positive Control
mouse brain , rat brain , PAX6-transfected 293T
Predict Reactivity
Rabbit, Bovine, Dog, Chicken, Pig, Xenopus tropicalis, Rhesus Monkey(>80% identity)
PROPERTIES
Form
Liquid
Buffer
PBS, 20% Glycerol
Preservative
0.025% ProClin 300
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
0.7 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Recombinant protein encompassing a sequence within the center region of human PAX6. The exact sequence is proprietary.
Purification
Purified by antigen-affinity chromatography.
Conjugation
Unconjugated
RRID
AB_1951119
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
paired box 6 , AN , AN2 , ASGD5 , D11S812E , FVH1 , MGDA , WAGR
Cellular Localization
Nucleus
Background
This gene encodes paired box gene 6, one of many human homologs of the Drosophila melanogaster gene prd. In addition to the hallmark feature of this gene family, a conserved paired box domain, the encoded protein also contains a homeo box domain. Both domains are known to bind DNA, and function as regulators of gene transcription. This gene is expressed in the developing nervous system, and in developing eyes. Mutations in this gene are known to cause ocular disorders such as aniridia and Peter's anomaly. Alternatively spliced transcript variants encoding either the same or different isoform have been found for this gene. [provided by RefSeq]
Database
Research Area
DATA IMAGES
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GTX113241 IHC-P Image
PAX6 antibody detects PAX6 protein at nucleus by immunohistochemical analysis.Sample: Paraffin-embedded mouse E10.5 embryo.Green: PAX6 stained by PAX6 antibody (GTX113241) diluted at 1:250.Red: beta Tubulin 3/ Tuj1, a cytoskeleton marker, stained by beta Tubulin 3/ Tuj1 antibody [GT11710] (GTX631836) diluted at 1:500.Blue: Fluoroshield with DAPI (GTX30920).Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GTX113241 IP Image
PAX6 antibody immunoprecipitates PAX6 protein in IP experiments. IP Sample: 1000 μg 293T whole cell lysate/extract A. 50 μg 293T whole cell lysate/extract B. Control with 2 μg of preimmune rabbit IgG C. Immunoprecipitation of PAX6 protein by 2 μg of PAX6 antibody (GTX113241) 10% SDS-PAGE The immunoprecipitated PAX6 protein was detected by PAX6 antibody (GTX113241) diluted at 1:1000. EasyBlot anti-rabbit IgG (GTX221666-01) was used as a secondary reagent.
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GTX113241 IHC-Fr Image
PAX6 antibody detects PAX6 protein expression by immunohistochemical analysis.
Sample: Frozen sectioned E13.5 Rat brain.
Green: PAX6 protein stained by PAX6 antibody (GTX113241) diluted at 1:250.
Red: beta Tubulin 3/ TUJ1, a mature neuron marker, stained by beta Tubulin 3/ TUJ1 antibody [GT11710] (GTX631836) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).
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GTX113241 IHC-P Image
PAX6 antibody detects PAX6 protein at nucleus by immunohistochemical analysis.Sample: Paraffin-embedded rat eye.Green: PAX6 stained by PAX6 antibody (GTX113241) diluted at 1:250.Red: beta Tubulin 3/ Tuj1, a neural marker, stained by beta Tubulin 3/ Tuj1 antibody [GT11710] (GTX631836) diluted at 1:500.Blue: Fluoroshield with DAPI (GTX30920).Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GTX113241 WB Image
Mouse tissue extract (50 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with PAX6 antibody (GTX113241) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX113241 ICC/IF Image
PAX6 antibody detects PAX6 protein at nucleus by immunofluorescent analysis.
Sample: SK-N-SH cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: PAX6 protein stained by PAX6 antibody (GTX113241) diluted at 1:500.
Red: Phalloidin, a cytoskeleton marker, diluted at 1:50.
Scale bar = 10 μm.
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GTX113241 ICC/IF Image
PAX6 antibody detects PAX6 protein at cytoplasm and nucleus by immunofluorescent analysis.Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: PAX6 stained by PAX6 antibody (GTX113241) diluted at 1:500.Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000.Blue: Fluoroshield with DAPI (GTX30920).
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GTX113241 IHC-Fr Image
PAX6 antibody detects PAX6 protein at nucleus in embryonic mouse brain by immunohistochemical analysis.
Sample: Frozen section of embryonic mouse brain (mE12.5).
PAX6 antibody (GTX113241) diluted at 1:250.
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GTX113241 WB Image
Rat tissue extract (50 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with PAX6 antibody (GTX113241) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX113241 WB Image
Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with PAX6 antibody (GTX113241) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX113241 IHC-P Image
PAX6 antibody detects PAX6 protein by immunohistochemical analysis.
Samples: Paraffin-Embedded mouse retina.
Green: PAX6 protein stained by PAX6 antibody (GTX113241) diluted at 1:250.
Red: beta Tubulin 3/ Tuj1, stained by beta Tubulin 3/ Tuj1 antibody [GT1338] (GTX631831) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GTX113241 IHC-P Image
PAX6 antibody detects PAX6 protein by immunohistochemical analysis.Sample: Paraffin-embedded mouse E10.5 embryo.Green: PAX6 stained by PAX6 antibody (GTX113241) diluted at 1:500.Red: beta Tubulin 3/ Tuj1, a Cytoskeleton marker, stained by beta Tubulin 3/ Tuj1 antibody [GT11710] (GTX631836) diluted at 1:500.Blue: Hoechst 33342 staining.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GTX113241 IHC-P Image
PAX6 antibody detects PAX6 protein at nucleus by immunohistochemical analysis.Sample: Paraffin-embedded mouse cerebellum.PAX6 stained by PAX6 antibody (GTX113241) diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GTX113241 IHC-P Image
PAX6 antibody detects PAX6 protein at nucleus by immunohistochemical analysis.Sample: Paraffin-embedded rat cerebellum.PAX6 stained by PAX6 antibody (GTX113241) diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GTX113241 WB Image
Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with PAX6 antibody (GTX113241) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.
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GTX113241 ICC/IF Image
The data was published in the journal Stem Cells Transl Med in 2017. PMID: 28191764
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GTX113241 ICC/IF Image
The data was published in the journal Stem Cells Transl Med in 2017. PMID: 28191764
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REFERENCE
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