GeneTex
United States (US)

PAX6 antibody

Overexpression
GTX113241_40100_IP_18121410_638.jpg
GTX113241_40100_20160921_IHC-Fr_18121410_520.jpg
GTX113241_41801_WB_18121410_865.jpg
GTX113241_40100_20160721_WB_R_brain_18121410_299.jpg
GTX113241_40100_20180727_WB_B_18121410_937.jpg
GTX113241_40100_20160721_WB_M_brain_18121410_265.jpg
GTX113241_40100_20161123_IFA_18121410_408.jpg
GTX113241_42823_20171017_IHC-P_M_18121410_839.jpg
GTX113241_40100_20150703_IHC-Fr_M_18121410_629.jpg

Cat No. GTX113241

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P, IHC-Fr, IP
Reactivity Human, Mouse, Rat
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IHC-Fr 1:100-1:1000
IP 1:500-1:1000
Not tested in other applications.

Calculated MW

47 kDa. ( Note )

Positive Control

293T , HeLa , mouse brain , rat brain , PAX6-transfected 293T

Predict Reactivity

Rabbit, Bovine, Dog, Chicken, Pig, Xenopus laevis, Rhesus Monkey(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.8mg/ml(Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human PAX6. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

AN antibody, AN2 antibody, D11S812E antibody, MGC17209 antibody, MGDA antibody, WAGR antibody, PAX6 antibody, paired box protein Pax-6 antibody, aniridia type II protein antibody, oculorhombin antibody, paired box homeotic gene-6 antibody, paired box 6 antibody

Cellular Localization

Nucleus

Background

This gene encodes paired box gene 6, one of many human homologs of the Drosophila melanogaster gene prd. In addition to the hallmark feature of this gene family, a conserved paired box domain, the encoded protein also contains a homeo box domain. Both domains are known to bind DNA, and function as regulators of gene transcription. This gene is expressed in the developing nervous system, and in developing eyes. Mutations in this gene are known to cause ocular disorders such as aniridia and Peter's anomaly. Alternatively spliced transcript variants encoding either the same or different isoform have been found for this gene. [provided by RefSeq]

Database

Research Area

DATA IMAGES
GTX113241_40100_IP_18121410_638.jpg

GTX113241 IP Image

PAX6 antibody immunoprecipitates PAX6 protein in IP experiments. IP Sample: 1000 µg 293T whole cell lysate/extract A. 50 μg 293T whole cell lysate/extract B. Control with 2 μg of preimmune rabbit IgG C. Immunoprecipitation of PAX6 protein by 2 μg of PAX6 antibody (GTX113241) 10% SDS-PAGE The immunoprecipitated PAX6 protein was detected by PAX6 antibody (GTX113241) diluted at 1:1000. EasyBlot anti-rabbit IgG (GTX221666-01) was used as a secondary reagent.

GTX113241_40100_20160921_IHC-Fr_18121410_520.jpg

GTX113241 IHC-Fr Image

PAX6 antibody detects PAX6 protein expression by immunohistochemical analysis.
Sample: Frozen sectioned E13.5 Rat brain.
Green: PAX6 protein stained by PAX6 antibody (GTX113241) diluted at 1:250.
Red: beta Tubulin 3/ TUJ1, a mature neuron marker, stained by beta Tubulin 3/ TUJ1 antibody [GT11710] (GTX631836) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

GTX113241_41801_WB_18121410_865.jpg

GTX113241 WB Image

PAX6 antibody detects PAX6 protein by Western blot analysis. Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with PAX6 antibody (GTX113241) diluted by 1:1000.

GTX113241_40100_20160721_WB_R_brain_18121410_299.jpg

GTX113241 WB Image

Rat tissue extract (50 µg) was separated by 10% SDS-PAGE, and the membrane was blotted with PAX6 antibody (GTX113241) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX113241_40100_20180727_WB_B_18121410_937.jpg

GTX113241 WB Image

Overexpression

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with PAX6 antibody (GTX113241) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX113241_40100_20160721_WB_M_brain_18121410_265.jpg

GTX113241 WB Image

Mouse tissue extract (50 µg) was separated by 10% SDS-PAGE, and the membrane was blotted with PAX6 antibody (GTX113241) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX113241_40100_20161123_IFA_18121410_408.jpg

GTX113241 ICC/IF Image

PAX6 antibody detects PAX6 protein at nucleus by immunofluorescent analysis.
Sample: SK-N-SH cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: PAX6 protein stained by PAX6 antibody (GTX113241) diluted at 1:500.
Red: Phalloidin, a cytoskeleton marker, diluted at 1:50.
Scale bar = 10 μm.

GTX113241_42823_20171017_IHC-P_M_18121410_839.jpg

GTX113241 IHC-P Image

PAX6 antibody detects PAX6 protein by immunohistochemical analysis.
Samples: Paraffin-Embedded mouse retina.
Green: PAX6 protein stained by PAX6 antibody (GTX113241) diluted at 1:250.
Red: beta Tubulin 3/ Tuj1, stained by beta Tubulin 3/ Tuj1 antibody [GT1338] (GTX631831) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX113241_40100_20150703_IHC-Fr_M_18121410_629.jpg

GTX113241 IHC-Fr Image

PAX6 antibody detects PAX6 protein at nucleus in embryonic mouse brain by immunohistochemical analysis.
Sample: Frozen section of embryonic mouse brain (mE12.5).
PAX6 antibody (GTX113241) diluted at 1:250.

REFERENCE
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 319
$ 169