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PAX6 antibody

Orthogonal
Anti-PAX6 antibody used in Immunoprecipitation (IP). GTX79189
Anti-PAX6 antibody used in Western Blot (WB). GTX79189
Anti-PAX6 antibody used in Western Blot (WB). GTX79189
Anti-PAX6 antibody used in Western Blot (WB). GTX79189
Anti-PAX6 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX79189

Cat. No. GTX79189

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IP

Reactivity

 Human, Mouse, Rat, Sheep, Primate
Package
100 μg ($399)
See all PAX6 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:1000
ICC/IF 1:50
IP 5 μg
Not tested in other applications.

Calculated MW

47 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 0.1% BSA

Preservative

0.05% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Mouse

Immunogen

Synthetic peptide corresponding to residues C R(267) E E K L R N Q R R Q A S N T P S H I(285) of mouse PAX6.

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

RRID

AB_11164868

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

paired box 6

Background

homeodomain (HD)-containing transcription factor; may play a role in the differentiation of the hindbrain somatic (SM) motoneurones and V1 interneurones in the hindbrain and/or spinal cord [RGD, Feb 2006]

Database

Research Area

DATA IMAGES

Anti-PAX6 antibody used in Immunoprecipitation (IP). GTX79189

GTX79189 IP Image

IP analysis of 293T cell lysates using GTX79189 PAX6 antibody.
IP reaction : 5μg antibody / 500μg lysate
Dilution : 1:1000

Anti-PAX6 antibody used in Western Blot (WB). GTX79189

GTX79189 WB Image

WB analysis of 50 ug of various human, mouse and non-human primate whole cell lysates per well using GTX79189 PAX6 antibody.
Dilution : 1:1000

Anti-PAX6 antibody used in Western Blot (WB). GTX79189

GTX79189 WB Image

WB analysis of rat whole eye extract using GTX79189 PAX6 antibody.

Anti-PAX6 antibody used in Western Blot (WB). GTX79189

GTX79189 WB Image

WB analysis of ovine retinal extract using GTX79189 PAX6 antibody.

Anti-PAX6 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX79189

GTX79189 ICC/IF Image

Orthogonal

ICC/IF analysis of All-Trans-Retinoic Acid (ATRA) treated (10μM, 24 hours) NCCIT cells using GTX79189 PAX6 antibody.
Green : Primary antibody
Blue : Nuclei
Red : Actin
Fixation : Formalin
Permeabilization : 0.1% Triton X-100 in TBS for 10 minutes
Dilution : 1:50 for at least 1 hour at room temperature

REFERENCE

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REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399