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PAX7 antibody [C3], C-term

Anti-PAX7 antibody [C3], C-term used in Western Blot (WB). GTX100702
Anti-PAX7 antibody [C3], C-term used in IHC (Paraffin sections) (IHC-P). GTX100702
Anti-PAX7 antibody [C3], C-term used in Western Blot (WB). GTX100702

Cat No. GTX100702

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Human, Mouse
Package
100 μl ($319),
25 μl ($169)
See all PAX7 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

57 kDa. ( Note )

Positive Control

293T , A431 , HeLaS3 , HepG2 , Molt-4 , Raji , mouse fetal brain

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human PAX7. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

paired box 7 , HUP1 , PAX7B , RMS2

Cellular Localization

Nucleus

Background

This gene is a member of the paired box (PAX) family of transcription factors. Members of this gene family typically contain a paired box domain, an octapeptide, and a paired-type homeodomain. These genes play critical roles during fetal development and cancer growth. The specific function of the paired box 7 gene is unknown but speculated to involve tumor suppression since fusion of this gene with a forkhead domain family member has been associated with alveolar rhabdomyosarcoma. Three transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-PAX7 antibody [C3], C-term used in Western Blot (WB). GTX100702

GTX100702 WB Image

Sample(30 ug whole cell lysate)
A:Hep G2 (GTX27900)
7.5% SDS PAGE
GTX100702 diluted at 1:1000

Anti-PAX7 antibody [C3], C-term used in IHC (Paraffin sections) (IHC-P). GTX100702

GTX100702 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human lung adenocarcinoma, using PAX7(GTX100702) antibody at 1:250 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-PAX7 antibody [C3], C-term used in Western Blot (WB). GTX100702

GTX100702 WB Image

Mouse tissue extract (50 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with PAX7 antibody [C3], C-term (GTX100702) diluted at 1:1000.

REFERENCE

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REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169