GeneTex
United States (US)

PAX7 antibody [PAX7/497]

Anti-PAX7 antibody [PAX7/497] used in Western Blot (WB). GTX34958

Cat No. GTX34958

Host Mouse
Clonality Monoclonal
Clone Name PAX7/497
Isotype IgG1
Application WB, ICC/IF, FACS
Reactivity Human, Mouse, Rat, Zebrafish, Chicken
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 0.5-1ug/ml
ICC/IF 0.5-1ug/ml
FACS Assay dependent
Not tested in other applications.

Calculated MW

57 kDa. ( Note )

Specificity/Sensitivity

The Pax gene family of nuclear transcription factors is comprised of nine members that function during embryogenesis to regulate the temporal and position-dependent differentiation of cells. In addition, the family is involved in a variety of signal transduction pathways in the adult organism. Mutations in the Pax family of proteins have been linked to disease and cancer in humans. Pax-7 is a protein specifically expressed in cultured satellite cell-derived myoblasts. In situ hybridization reveals that Pax-7 is also expressed in satellite cells residing in adult muscle. A chromosomal aberration in the gene encoding Pax-7 causes rhabdomyosarcoma 2 (RMS2) (also called alveolar rhabdomyosarcoma).
PROPERTIES

Form

Liquid

Buffer

Prepared in 10mM PBS with 0.05% BSA and 0.05% azide.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Antigen Species

Human

Immunogen

Recombinant fragment (aa301-505) of human PAX7 protein (exact sequence is proprietary)

Purification

Ab purified from Bioreactor Concentrate by Protein A/G

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Paired Box 7,Hup1,Pax7B,Rms2,Pax7

Background

This gene is a member of the paired box (PAX) family of transcription factors. Members of this gene family typically contain a paired box domain, an octapeptide, and a paired-type homeodomain. These genes play critical roles during fetal development and cancer growth. The specific function of the paired box 7 gene is unknown but speculated to involve tumor suppression since fusion of this gene with a forkhead domain family member has been associated with alveolar rhabdomyosarcoma. Three transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2008]

Database

Research Area

DATA IMAGES
Anti-PAX7 antibody [PAX7/497] used in Western Blot (WB). GTX34958

GTX34958 WB Image

Western Blot of HeLa Cell Lysate using PAX7 Monoclonal Antibody (PAX7/497).

Package List Price ($)
$ 329