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PAX7 antibody

Anti-PAX7 antibody used in Western Blot (WB). GTX04358
Anti-PAX7 antibody used in Western Blot (WB). GTX04358
Anti-PAX7 antibody used in Western Blot (WB). GTX04358
Anti-PAX7 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX04358
Anti-PAX7 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX04358

Cat. No. GTX04358

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF

Reactivity

 Human, Mouse
Package
100 μl ($399)
See all PAX7 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:2000
ICC/IF 1:100-1:500
Not tested in other applications.

Calculated MW

55 kDa. ( Note )

Predict Reactivity

Rat, Rabbit, Sheep, Dog, Chicken, Pig, Xenopus(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 150mM NaCl, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

A synthesized peptide derived from human PAX7(Accession P23759), corresponding to amino acid residues D178-Q228.

Purification

Purified by antigen-affinity chromatography
From serum

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

paired box 7 , HUP1 , PAX7B , RMS2

Cellular Localization

Nucleus

Background

This gene is a member of the paired box (PAX) family of transcription factors. Members of this gene family typically contain a paired box domain, an octapeptide, and a paired-type homeodomain. These genes play critical roles during fetal development and cancer growth. The specific function of the paired box 7 gene is unknown but speculated to involve tumor suppression since fusion of this gene with a forkhead domain family member has been associated with alveolar rhabdomyosarcoma. Three transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2008]

Database

Research Area

DATA IMAGES

Anti-PAX7 antibody used in Western Blot (WB). GTX04358

GTX04358 WB Image

WB analysis of mouse brain tissue tissue lysate using GTX04358 PAX7 antibody. The lane on the left was treated with blocking peptide.

Anti-PAX7 antibody used in Western Blot (WB). GTX04358

GTX04358 WB Image

WB analysis of mouse brain tissue tissue lysate using GTX04358 PAX7 antibody. The lane on the left was treated with blocking peptide.

Anti-PAX7 antibody used in Western Blot (WB). GTX04358

GTX04358 WB Image

WB analysis of HeLa whole cell lysate using GTX04358 PAX7 antibody. The lane on the right was treated with blocking peptide.

Anti-PAX7 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX04358

GTX04358 ICC/IF Image

ICC/IF analysis of HeLa cells using GTX04358 PAX7 antibody.
Red : Primnry antibody
Blue : Nucleus
Dilution : 1:100

Anti-PAX7 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX04358

GTX04358 ICC/IF Image

ICC/IF analysis of PFA fixed HeLa cells using GTX04358 PAX7 antibody.
Red : Primary antibody
Green : Beta tubulin
Blue : DAPI
Permeabilization : 0.1% Triton X-100
Dilution : 1:200

REFERENCE

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REVIEW

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Package List Price ($)
$ 399