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PAX7 antibody

Overexpression
Anti-PAX7 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX54533
Anti-PAX7 antibody used in Western Blot (WB). GTX54533
Anti-PAX7 antibody used in Immunoprecipitation (IP). GTX54533
Anti-PAX7 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX54533
Anti-PAX7 antibody used in Western Blot (WB). GTX54533
Anti-PAX7 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX54533
Anti-PAX7 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX54533

Cat. No. GTX54533

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IP

Reactivity

 Human, Mouse
Package
100 μg ($399)
See all PAX7 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:1000 - 1:2000
ICC/IF 1:20-1:200
IP 3 μg
Not tested in other applications.

Calculated MW

55 kDa. ( Note )

Product Note

This antibody detects a prominent ~54 kDa protein in human HeLa, MCF7, rhabdomyosarcoma and skeletal muscle satellite cells and a weaker ~57 kDa protein in HeLa, rhabdomyosarcoma and skeletal muscle satellite cells. The ~57 kDa band seems to be enriched in immunoprecipitations from HeLa lysate, suggesting a bias for the larger isoform in ths application. Weak unknown bands of lower molecular weight and at ~110-130 kDa are also detected. Specificity of the antibody was also confirmed in Hela cells overexpressing full length PAX7.

PROPERTIES

Form

Liquid

Buffer

PBS, 30% Glycerol, 1mg/ml BSA

Preservative

0.05% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Purified internal fragment of human recombinant PAX7 expressed in E. coli.

Purification

Protein G purified

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

paired box 7 , HUP1 , PAX7B , RMS2

Cellular Localization

Nucleus

Background

This gene is a member of the paired box (PAX) family of transcription factors. Members of this gene family typically contain a paired box domain, an octapeptide, and a paired-type homeodomain. These genes play critical roles during fetal development and cancer growth. The specific function of the paired box 7 gene is unknown but speculated to involve tumor suppression since fusion of this gene with a forkhead domain family member has been associated with alveolar rhabdomyosarcoma. Three transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2008]

Database

Research Area

DATA IMAGES

Anti-PAX7 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX54533

GTX54533 ICC/IF Image

ICC/IF analysis of MCF7 and HeLa cells using GTX54533 PAX7 antibody.
Green : Primary antibody
Blue : Nuclei
Red : Actin
Fixation : Formalin
Permeabilization : 0.1% Triton X-100 in TBS for 10 minutes
Dilution : 1:100 for at least 1 hour at room temperature

Anti-PAX7 antibody used in Western Blot (WB). GTX54533

GTX54533 WB Image

Overexpression

WB analysis of 50μg of HeLa lysate overexpressing PAX7 (right lane) or empty vector control (left lane) using GTX54533 PAX7 antibody.
Dilution : 1:1000

Anti-PAX7 antibody used in Immunoprecipitation (IP). GTX54533

GTX54533 IP Image

IP analysis of HeLa cell lysates using GTX54533 PAX7 antibody.
IP reaction : 3μg antibody / 500μg lysate
Dilution : 1:1000

Anti-PAX7 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX54533

GTX54533 ICC/IF Image

ICC/IF analysis of C2C12 cells using GTX54533 PAX7 antibody. Cells were probed without (left) or with(right) an antibody.
Green : Primary antibody
Blue : Nuclei
Red : Actin
Fixation : Formalin
Permeabilization : 0.1% Triton X-100 in TBS for 5-10 minute
Dilution : 1:100 and incubated overnight at 4ºC

Anti-PAX7 antibody used in Western Blot (WB). GTX54533

GTX54533 WB Image

WB analysis of 50 μg of various whole cell lysates using GTX54533 PAX7 antibody.
Dilution : 1:1000

Anti-PAX7 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX54533

GTX54533 ICC/IF Image

ICC/IF analysis of HeLa cells using GTX54533 PAX7 antibody. Cells were probed without (left) or with(right) an antibody.
Green : Primary antibody
Blue : Nuclei
Red : Actin
Fixation : Formalin
Permeabilization : 0.1% Triton X-100 in TBS for 5-10 minute
Dilution : 1:100 and incubated overnight at 4ºC

Anti-PAX7 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX54533

GTX54533 ICC/IF Image

ICC/IF analysis of MCF-7 cells using GTX54533 PAX7 antibody. Cells were probed without (left) or with(right) an antibody.
Green : Primary antibody
Blue : Nuclei
Red : Actin
Fixation : Formalin
Permeabilization : 0.1% Triton X-100 in TBS for 5-10 minute
Dilution : 1:100 and incubated overnight at 4ºC

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399