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PAX8 antibody [1F8-3A8]

Overexpression
Anti-PAX8 antibody [1F8-3A8] used in IHC (Paraffin sections) (IHC-P). GTX31119
Anti-PAX8 antibody [1F8-3A8] used in IHC (Paraffin sections) (IHC-P). GTX31119
Anti-PAX8 antibody [1F8-3A8] used in IHC (Paraffin sections) (IHC-P). GTX31119
Anti-PAX8 antibody [1F8-3A8] used in Western Blot (WB). GTX31119
Anti-PAX8 antibody [1F8-3A8] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX31119
Anti-PAX8 antibody [1F8-3A8] used in Western Blot (WB). GTX31119
Anti-PAX8 antibody [1F8-3A8] used in Western Blot (WB). GTX31119

Cat. No. GTX31119

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 1F8-3A8

Isotype

 IgG1

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse, Primate
Package
100 μg ($399)
See all PAX8 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500 - 1:2000
ICC/IF 1:50-1:200
IHC-P 1:20-1:200
Not tested in other applications.

Calculated MW

48 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 1mg/ml BSA, 30% Glycerol

Preservative

0.05% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Purified internal fragment of human recombinant PAX8 expressed in E. coli.

Purification

Protein A purified

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

paired box 8

Cellular Localization

Nucleus

Background

This gene encodes a member of the paired box (PAX) family of transcription factors. Members of this gene family typically encode proteins that contain a paired box domain, an octapeptide, and a paired-type homeodomain. This nuclear protein is involved in thyroid follicular cell development and expression of thyroid-specific genes. Mutations in this gene have been associated with thyroid dysgenesis, thyroid follicular carcinomas and atypical follicular thyroid adenomas. Alternatively spliced transcript variants encoding different isoforms have been described. [provided by RefSeq, Mar 2010]

Database

Research Area

DATA IMAGES

Anti-PAX8 antibody [1F8-3A8] used in IHC (Paraffin sections) (IHC-P). GTX31119

GTX31119 IHC-P Image

IHC-P analysis of human kidney tissue using GTX31119 PAX8 antibody [1F8-3A8].
Right : Primary antibody
Left : Negative control without primary antibody
Antigen retrieval : 10mM sodium citrate (pH 6.0), microwaved for 8-15 min
Dilution : 1:100

Anti-PAX8 antibody [1F8-3A8] used in IHC (Paraffin sections) (IHC-P). GTX31119

GTX31119 IHC-P Image

IHC-P analysis of mouse kidney tissue using GTX31119 PAX8 antibody [1F8-3A8].
Right : Primary antibody
Left : Negative control without primary antibody
Antigen retrieval : 10mM sodium citrate (pH 6.0), microwaved for 8-15 min
Dilution : 1:20

Anti-PAX8 antibody [1F8-3A8] used in IHC (Paraffin sections) (IHC-P). GTX31119

GTX31119 IHC-P Image

IHC-P analysis of human thyroid tissue using GTX31119 PAX8 antibody [1F8-3A8].
Right : Primary antibody
Left : Negative control without primary antibody
Antigen retrieval : 10mM sodium citrate (pH 6.0), microwaved for 8-15 min
Dilution : 1:100

Anti-PAX8 antibody [1F8-3A8] used in Western Blot (WB). GTX31119

GTX31119 WB Image

WB analysis of 60 ug of SKOV-3, COS7, and negative control HeLa whole cell lysates using GTX31119 PAX8 antibody [1F8-3A8].
Dilution : 1:1000

Anti-PAX8 antibody [1F8-3A8] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX31119

GTX31119 ICC/IF Image

ICC/IF analysis of 293T (left panel) and COS7 cells (right panel) using GTX31119 PAX8 antibody [1F8-3A8].
Green : PAX8
Red: F-actin
Blue: Hoechst 33342
Fixation : Formalin
Permeabilization : 0.1% Triton X-100 in TBS for 10 minutes
Dilution : 1:50 for at least 1 hour at room temperature

Anti-PAX8 antibody [1F8-3A8] used in Western Blot (WB). GTX31119

GTX31119 WB Image

Overexpression

WB analysis of 25 ug of HEK293T cell lysate overexpressing PAX8 (right lane) or empty vector control (left lane) using GTX31119 PAX8 antibody [1F8-3A8].
Dilution : 1:1000

Anti-PAX8 antibody [1F8-3A8] used in Western Blot (WB). GTX31119

GTX31119 WB Image

WB analysis of 20 ug of the indicated whole cell lysates using GTX31119 PAX8 antibody [1F8-3A8].
Dilution : 1:500

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399