GeneTex
United States (US)

PAX8 antibody [1F8-3A8]

Overexpression
GTX31119_2082_WB_19011519_757.jpg
GTX31119_732_ICC-IF_19011519_648.jpg
GTX31119_1336_IHC-P_19011519_192.jpg
GTX31119_1337_IHC-P_19011519_219.jpg
GTX31119_1845_WB_19011519_668.jpg
GTX31119_1335_IHC-P_19011519_529.jpg
GTX31119_1846_WB_19011519_211.jpg

Cat No. GTX31119

Host Mouse
Clonality Monoclonal
Clone Name 1F8-3A8
Isotype IgG1
Application WB, ICC/IF, IHC-P
Reactivity Human, Mouse, Primate
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500 - 1:2000
ICC/IF 1:50-1:200
IHC-P 1:20-1:200
Not tested in other applications.

Calculated MW

48 kDa. ( Note )
PROPERTIES

Form

Liquid

Buffer

PBS, 1mg/ml BSA, 30% glycerol, 0.05% sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1mg/ml(Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Purified internal fragment of human recombinant PAX8 expressed in E. coli.

Purification

Protein A purified

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Cellular Localization

Nucleus

Background

This gene encodes a member of the paired box (PAX) family of transcription factors. Members of this gene family typically encode proteins that contain a paired box domain, an octapeptide, and a paired-type homeodomain. This nuclear protein is involved in thyroid follicular cell development and expression of thyroid-specific genes. Mutations in this gene have been associated with thyroid dysgenesis, thyroid follicular carcinomas and atypical follicular thyroid adenomas. Alternatively spliced transcript variants encoding different isoforms have been described. [provided by RefSeq, Mar 2010]

Database

Research Area

DATA IMAGES
GTX31119_2082_WB_19011519_757.jpg

GTX31119 WB Image

Overexpression

WB analysis of 25 ug of HEK293T cell lysate overexpressing PAX8 (right lane) or empty vector control (left lane) using GTX31119 PAX8 antibody [1F8-3A8].
Dilution : 1:1000

GTX31119_732_ICC-IF_19011519_648.jpg

GTX31119 ICC/IF Image

ICC/IF analysis of 293T (left panel) and COS7 cells (right panel) using GTX31119 PAX8 antibody [1F8-3A8].
Green : PAX8
Red: F-actin
Blue: Hoechst 33342
Fixation : Formalin
Permeabilization : 0.1% Triton X-100 in TBS for 10 minutes
Dilution : 1:50 for at least 1 hour at room temperature

GTX31119_1336_IHC-P_19011519_192.jpg

GTX31119 IHC-P Image

IHC-P analysis of human thyroid tissue using GTX31119 PAX8 antibody [1F8-3A8].
Right : Primary antibody
Left : Negative control without primary antibody
Antigen retrieval : 10mM sodium citrate (pH 6.0), microwaved for 8-15 min
Dilution : 1:100

GTX31119_1337_IHC-P_19011519_219.jpg

GTX31119 IHC-P Image

IHC-P analysis of mouse kidney tissue using GTX31119 PAX8 antibody [1F8-3A8].
Right : Primary antibody
Left : Negative control without primary antibody
Antigen retrieval : 10mM sodium citrate (pH 6.0), microwaved for 8-15 min
Dilution : 1:20

GTX31119_1845_WB_19011519_668.jpg

GTX31119 WB Image

WB analysis of 20 ug of the indicated whole cell lysates using GTX31119 PAX8 antibody [1F8-3A8].
Dilution : 1:500

GTX31119_1335_IHC-P_19011519_529.jpg

GTX31119 IHC-P Image

IHC-P analysis of human kidney tissue using GTX31119 PAX8 antibody [1F8-3A8].
Right : Primary antibody
Left : Negative control without primary antibody
Antigen retrieval : 10mM sodium citrate (pH 6.0), microwaved for 8-15 min
Dilution : 1:100

GTX31119_1846_WB_19011519_211.jpg

GTX31119 WB Image

WB analysis of 60 ug of SKOV-3, COS7, and negative control HeLa whole cell lysates using GTX31119 PAX8 antibody [1F8-3A8].
Dilution : 1:1000