WB analysis of 20 ug of the indicated whole cell lysates using GTX31119 PAX8 antibody [1F8-3A8].
Dilution : 1:500

WB analysis of 25 ug of HEK293T cell lysate overexpressing PAX8 (right lane) or empty vector control (left lane) using GTX31119 PAX8 antibody [1F8-3A8].
Dilution : 1:1000

ICC/IF analysis of 293T (left panel) and COS7 cells (right panel) using GTX31119 PAX8 antibody [1F8-3A8].
Green : PAX8
Red: F-actin
Blue: Hoechst 33342
Fixation : Formalin
Permeabilization : 0.1% Triton X-100 in TBS for 10 minutes
Dilution : 1:50 for at least 1 hour at room temperature

WB analysis of 60 ug of SKOV-3, COS7, and negative control HeLa whole cell lysates using GTX31119 PAX8 antibody [1F8-3A8].
Dilution : 1:1000

IHC-P analysis of human thyroid tissue using GTX31119 PAX8 antibody [1F8-3A8].
Right : Primary antibody
Left : Negative control without primary antibody
Antigen retrieval : 10mM sodium citrate (pH 6.0), microwaved for 8-15 min
Dilution : 1:100

IHC-P analysis of mouse kidney tissue using GTX31119 PAX8 antibody [1F8-3A8].
Right : Primary antibody
Left : Negative control without primary antibody
Antigen retrieval : 10mM sodium citrate (pH 6.0), microwaved for 8-15 min
Dilution : 1:20

IHC-P analysis of human kidney tissue using GTX31119 PAX8 antibody [1F8-3A8].
Right : Primary antibody
Left : Negative control without primary antibody
Antigen retrieval : 10mM sodium citrate (pH 6.0), microwaved for 8-15 min
Dilution : 1:100