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PAX8 antibody, N-term

Anti-PAX8 antibody, N-term used in IHC (Paraffin sections) (IHC-P). GTX13611
Anti-PAX8 antibody, N-term used in Western Blot (WB). GTX13611

Cat. No. GTX13611

Host

 Goat

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Human
Package
100 μg ($399)
See all PAX8 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1-2μg/ml
IHC-P Assay dependent
Not tested in other applications.

Calculated MW

48 kDa. ( Note )

Positive Control

The peptide used to generate this antibody is available for purchase (GTX13611-PEP).

Product Note

This antibody is expected to recognise all five human isoforms (as represented by NP_003457.1; NP_039245.1; NP_039246.1; NP_039247.1; NP_054698.1)

Predict Reactivity

Mouse, Rat(>80% identity)

PROPERTIES

Form

Liquid

Buffer

TBS, 0.5% BSA

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.50 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Peptide with sequence PHNSIRSGHGGLN-C, from the N Terminus of the protein sequence according to NP_003457.1; NP_039245.1; NP_039246.1; NP_039247.1; NP_054698.1.

Purification

Purified by ammonium sulphate precipitation followed by antigen affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

paired box 8

Cellular Localization

Nucleus

Background

This gene encodes a member of the paired box (PAX) family of transcription factors. Members of this gene family typically encode proteins that contain a paired box domain, an octapeptide, and a paired-type homeodomain. This nuclear protein is involved in thyroid follicular cell development and expression of thyroid-specific genes. Mutations in this gene have been associated with thyroid dysgenesis, thyroid follicular carcinomas and atypical follicular thyroid adenomas. Alternatively spliced transcript variants encoding different isoforms have been described. [provided by RefSeq, Mar 2010]

Database

Research Area

DATA IMAGES

Anti-PAX8 antibody, N-term used in IHC (Paraffin sections) (IHC-P). GTX13611

GTX13611 IHC-P Image

IHC-P analysis of human thyroid Gland using GTX13611 PAX8 antibody, N-term.
Antigen retrieval : citrate buffer pH 6
Dilution : 4μg/ml

Anti-PAX8 antibody, N-term used in Western Blot (WB). GTX13611

GTX13611 WB Image

WB analysis of human kidney (A) and HeLa cell (B) lysate using GTX13611 PAX8 antibody, N-term.
Dilution : 1μg/ml
Loading : 35μg protein in RIPA buffer

REFERENCE

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REVIEW

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SDS
Sodium Azide.pdf
Package List Price ($)
$ 399