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PAX8 antibody [PAX8/1492]

Anti-PAX8 antibody [PAX8/1492] used in IHC (Paraffin sections) (IHC-P). GTX34962
Anti-PAX8 antibody [PAX8/1492] used in IHC (Paraffin sections) (IHC-P). GTX34962
Anti-PAX8 antibody [PAX8/1492] used in Western Blot (WB). GTX34962
Anti-PAX8 antibody [PAX8/1492] used in IHC (Paraffin sections) (IHC-P). GTX34962

Cat No. GTX34962

Host Mouse
Clonality Monoclonal
Clone Name PAX8/1492
Isotype IgG2a
Application WB, ICC/IF, IHC-P
Reactivity Human
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 0.5-1ug/ml
ICC/IF 1-2ug/ml
IHC-P 1-2ug/ml for 30 minutes at RT

Note :

IHC-P
Boiling tissue sections in 10mM Tris buffer with 1mM EDTA, pH 9.0, for 10-20 min followed by cooling at RT for 20 minutes

Not tested in other applications.

Calculated MW

48 kDa. ( Note )

Specificity/Sensitivity

Recognizes a protein of 62kDa, identified as PAX8. It is a member of the paired box (PAX) family of transcription factors. This nuclear protein is involved in thyroid follicular cell development and expression of thyroid-specific genes. Mutations in this gene have been associated with thyroid dysgenesis, thyroid follicular carcinomas, and atypical thyroid adenomas. PAX-8 is expressed in the thyroid (and associated carcinomas), non-ciliated mucosal cells of the fallopian tubes, and simple ovarian inclusion cysts, but not normal ovarian surface epithelial cells. PAX-8 is expressed in a high percentage of ovarian serous, endometrioid, and clear cell carcinomas, but only rarely in primary ovarian mucinous adenocarcinomas. PAX-8 expression is reported in renal tubules as well as renal cell carcinoma, nephroblastoma, and seminoma. PAX-8 antibody may be used as an additional immunohistochemical marker for renal epithelial tumors.
PROPERTIES

Form

Liquid

Buffer

Prepared in 10mM PBS with 0.05% BSA and 0.05% azide.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Antigen Species

Human

Immunogen

Recombinant human PAX8 fragment (aa60-261) (exact sequence is proprietary)

Purification

Ab purified from Bioreactor Concentrate by Protein A/G

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Paired Box 8,Pax8

Background

This gene encodes a member of the paired box (PAX) family of transcription factors. Members of this gene family typically encode proteins that contain a paired box domain, an octapeptide, and a paired-type homeodomain. This nuclear protein is involved in thyroid follicular cell development and expression of thyroid-specific genes. Mutations in this gene have been associated with thyroid dysgenesis, thyroid follicular carcinomas and atypical follicular thyroid adenomas. Alternatively spliced transcript variants encoding different isoforms have been described. [provided by RefSeq, Mar 2010]

Database

Research Area

DATA IMAGES
Anti-PAX8 antibody [PAX8/1492] used in IHC (Paraffin sections) (IHC-P). GTX34962

GTX34962 IHC-P Image

Formalin-fixed, paraffin-embedded human Urothelial Carcinoma stained with PAX8 Monoclonal Antibody (PAX8/1492).

Anti-PAX8 antibody [PAX8/1492] used in IHC (Paraffin sections) (IHC-P). GTX34962

GTX34962 IHC-P Image

Formalin-fixed, paraffin-embedded human Thyroid Carcinoma stained with PAX8 Monoclonal Antibody (PAX8/1492).

Anti-PAX8 antibody [PAX8/1492] used in Western Blot (WB). GTX34962

GTX34962 WB Image

Western Blot Analysis (A) Recombinant Protein (B) Raji Cell Lysate Using PAX8 Monoclonal Antibody (PAX8/1492).

Anti-PAX8 antibody [PAX8/1492] used in IHC (Paraffin sections) (IHC-P). GTX34962

GTX34962 IHC-P Image

Formalin-fixed, paraffin-embedded human Renal Cell Carcinoma stained with PAX8 Monoclonal Antibody (PAX8/1492).