GeneTex
United States (US)

PAX8 antibody

Orthogonal Overexpression
GTX101583_39967_ChIP_19011506_502.jpg
GTX101583_39967_IP_19011506_741.jpg
GTX101583_42634_20161110_IHC-P_M_19011506_673.jpg
GTX101583_42613_20180608_WB_B_19011506_978.jpg
GTX101583_42613_20161027_WB_Fraction_19011506_361.jpg
GTX101583_39967_20151223_IHC-P_19011506_126.jpg
GTX101583_39967_IHC_R_19011506_652.jpg
GTX101583_IFA_19011506_786.jpg

Cat No. GTX101583

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P, IP, ChIP assay
Reactivity Human, Mouse, Rat
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:5000-1:20000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IP 1:500-1:1000
ChIP assay Assay dependent
Not tested in other applications.

Calculated MW

48 kDa. ( Note )

Positive Control

293T , 293T nuclear extract , DDDDK-tagged PAX8-transfected 293T

Predict Reactivity

Dog, Xenopus laevis(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.87mg/ml

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human PAX8. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

PAX8 antibody, paired domain gene 8 antibody, paired box protein Pax-8 antibody, paired box 8 antibody

Cellular Localization

Nucleus

Background

This gene encodes a member of the paired box (PAX) family of transcription factors. Members of this gene family typically encode proteins that contain a paired box domain, an octapeptide, and a paired-type homeodomain. This nuclear protein is involved in thyroid follicular development and expression of thyroid-specific genes. Mutations in this gene have been associated with thyroid dysgenesis, thyroid follicular carcinomas and atypical follicular thyroid adenomas. Alternateively spliced transcript variants encoding different isoforms have been described. [provided by RefSeq]
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Database

Research Area

DATA IMAGES
GTX101583_39967_ChIP_19011506_502.jpg

GTX101583 ChIP assay Image

PAX8 antibody immunoprecipitates PAX8 protein-DNA in ChIP experiments. ChIP Sample: 293T whole cell lysate/extract A. 5 μg preimmune rabbit IgG B. 5 μg of PAX8 antibody (GTX101583) The precipitated DNA was detected by PCR with primer set targeting to E2F1 promoter.

GTX101583_39967_IP_19011506_741.jpg

GTX101583 IP Image

PAX8 antibody immunoprecipitates PAX8 protein in IP experiments. IP Sample: 1000 µg 293T whole cell lysate/extract A. 40 μg 293T whole cell lysate/extract B. Control with 2 μg of preimmune rabbit IgG C. Immunoprecipitation of PAX8 protein by 2 μg of PAX8 antibody (GTX101583) 10% SDS-PAGE The immunoprecipitated PAX8 protein was detected by PAX8 antibody (GTX101583) diluted at 1:1000. EasyBlot anti-rabbit IgG (GTX221666-01) was used as a secondary reagent.

GTX101583_42634_20161110_IHC-P_M_19011506_673.jpg

GTX101583 IHC-P Image

PAX8 antibody detects PAX8 protein at nucleus in mouse liver by immunohistochemical analysis.
Sample: Paraffin-embedded mouse liver.
PAX8 antibody (GTX101583) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX101583_42613_20180608_WB_B_19011506_978.jpg

GTX101583 WB Image

Overexpression

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with PAX8 antibody (GTX101583) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX101583_42613_20161027_WB_Fraction_19011506_361.jpg

GTX101583 WB Image

Orthogonal

293T whole cell and nuclear extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with PAX8 antibody (GTX101583) diluted at 1:1000.

GTX101583_39967_20151223_IHC-P_19011506_126.jpg

GTX101583 IHC-P Image

PAX8 antibody detects PAX8 protein at nucleolus by immunohistochemical analysis.
Sample: Paraffin-embedded DLD1 xenograft .
PAX8 antibody (GTX101583) diluted at 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX101583_39967_IHC_R_19011506_652.jpg

GTX101583 IHC-P Image

PAX8 antibody detects PAX8 protein at cytosol and nucleus on rat thyroid gland by immunohistochemical analysis.
Sample: Paraffin-embedded rat thyroid gland.
PAX8 antibody (GTX101583) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX101583_IFA_19011506_786.jpg

GTX101583 ICC/IF Image

Immunofluorescence analysis of paraformaldehyde-fixed A549, using PAX8(GTX101583) antibody at 1:200 dilution.

SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf