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PB1 antibody [N1N2], N-term

Anti-PB1 antibody [N1N2], N-term used in IHC (Paraffin sections) (IHC-P). GTX100781
Anti-PB1 antibody [N1N2], N-term used in Western Blot (WB). GTX100781
Anti-PB1 antibody [N1N2], N-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX100781

Cat. No. GTX100781

Reference 2

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse
Package
100 μl ($399),
25 μl ($169)
See all PB1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

193 kDa. ( Note )

Positive Control

A431

Predict Reactivity

Bovine, Chicken(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the N-terminus region of human PB1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1241159

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

polybromo 1 , BAF180 , PB1

Cellular Localization

Nucleus

Background

Involved in transcriptional activation and repression of select genes by chromatin remodeling (alteration of DNA-nucleosome topology).

Database

Research Area

DATA IMAGES

Anti-PB1 antibody [N1N2], N-term used in IHC (Paraffin sections) (IHC-P). GTX100781

GTX100781 IHC-P Image

PB1 antibody [N1N2], N-term detects PB1 protein at nucleus in mouse duodenum by immunohistochemical analysis.
Sample: Paraffin-embedded mouse duodenum.
PB1 antibody [N1N2], N-term (GTX100781) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-PB1 antibody [N1N2], N-term used in Western Blot (WB). GTX100781

GTX100781 WB Image

Sample (30 μg of whole cell lysate)
A: A431 (GTX27909)
5% SDS PAGE
PB1 antibody
GTX100781 diluted at 1:500
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-PB1 antibody [N1N2], N-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX100781

GTX100781 ICC/IF Image

Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using PB1(GTX100781) antibody at 1:500 dilution.

REFERENCE

Application Reference

Bdeir N et al. Sci Rep 2021; 11 (1) : 20477 Evidence that two instead of one defective interfering RNA in influenza A virus-derived defective interfering particles (DIPs) does not enhance antiviral activity.
Application : WB
Wang SA et al. Sci Rep 2020; 10 (1) : 20870 USP24 stabilizes bromodomain containing proteins to promote lung cancer malignancy.
Application : WB
Reactivity : Human
Submit a Reference

REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169