GeneTex
United States (US)

PB1 antibody [N1N2], N-term

GTX100781_WB_19011506_607.jpg
GTX100781_39549_20160222_IHC-P_M_19011506_151.jpg
GTX100781_39549_IFA_19011506_839.jpg

Cat No. GTX100781

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P
Reactivity Human, Mouse
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

193 kDa. ( Note )

Positive Control

A431

Predict Reactivity

Bovine, Chicken(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1mg/ml(Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the N-terminus region of human PB1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

BAF180 antibody, MGC156155 antibody, MGC156156 antibody, PB1 antibody, PBRM1 antibody, protein polybromo-1 antibody, polybromo-1D antibody, BRG1-associated factor 180 antibody, polybromo 1 antibody

Cellular Localization

Nucleus

Background

Involved in transcriptional activation and repression of select genes by chromatin remodeling (alteration of DNA-nucleosome topology).

Database

Research Area

DATA IMAGES
GTX100781_WB_19011506_607.jpg

GTX100781 WB Image

Sample (30 µg of whole cell lysate)
A: A431 (GTX27909)
5% SDS PAGE
PB1 antibody
GTX100781 diluted at 1:500
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX100781_39549_20160222_IHC-P_M_19011506_151.jpg

GTX100781 IHC-P Image

PB1 antibody [N1N2], N-term detects PB1 protein at nucleus in mouse duodenum by immunohistochemical analysis.
Sample: Paraffin-embedded mouse duodenum.
PB1 antibody [N1N2], N-term (GTX100781) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX100781_39549_IFA_19011506_839.jpg

GTX100781 ICC/IF Image

Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using PB1(GTX100781) antibody at 1:500 dilution.

SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169