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PBK antibody [2C8]

Anti-PBK antibody [2C8] used in IHC (Paraffin sections) (IHC-P). GTX60560
Anti-PBK antibody [2C8] used in Western Blot (WB). GTX60560
Anti-PBK antibody [2C8] used in IHC (Paraffin sections) (IHC-P). GTX60560
Anti-PBK antibody [2C8] used in Western Blot (WB). GTX60560
Anti-PBK antibody [2C8] used in ELISA (ELISA). GTX60560
Anti-PBK antibody [2C8] used in Flow cytometry (FACS). GTX60560
Anti-PBK antibody [2C8] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX60560

Cat. No. GTX60560

Reference 1

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 2C8

Isotype

 IgG2b

Application

 WB, ICC/IF, IHC-P, FACS, ELISA

Reactivity

 Human
Package
100 μl ($399)
See all PBK products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1/500 - 1/2000
ICC/IF 1/200 - 1/1000
IHC-P 1/200 - 1/1000
FACS 1/200 - 1/400
ELISA 1/10000
Not tested in other applications.

Calculated MW

36 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

Ascites

Preservative

0.03% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Antigen Species

Human

Immunogen

Purified recombinant fragment of human PBK expressed in E. Coli.

Purification

Unpurified

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

PDZ binding kinase , CT84 , HEL164 , Nori-3 , SPK , TOPK

Background

This genes encodes a serine/threonine kinase related to the dual specific mitogen-activated protein kinase kinase (MAPKK) family. Evidence suggests that mitotic phosphorylation is required for its catalytic activity. This mitotic kinase may be involved in the activation of lymphoid cells and support testicular functions, with a suggested role in the process of spermatogenesis. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-PBK antibody [2C8] used in IHC (Paraffin sections) (IHC-P). GTX60560

GTX60560 IHC-P Image

IHC-P analysis of ovarian cancer tissue using GTX60560 PBK antibody [2C8].

Anti-PBK antibody [2C8] used in Western Blot (WB). GTX60560

GTX60560 WB Image

WB analysis of A431 cell lysate using GTX60560 PBK antibody [2C8].

Anti-PBK antibody [2C8] used in IHC (Paraffin sections) (IHC-P). GTX60560

GTX60560 IHC-P Image

IHC-P analysis of colon cancer tissue using GTX60560 PBK antibody [2C8].

Anti-PBK antibody [2C8] used in Western Blot (WB). GTX60560

GTX60560 WB Image

WB analysis of human PBK (AA: 50-230) recombinant protein using GTX60560 PBK antibody [2C8].

Anti-PBK antibody [2C8] used in ELISA (ELISA). GTX60560

GTX60560 ELISA Image

ELISA analysis of antigen using GTX60560 PBK antibody [2C8].
Black : Control antigen 100ng
Purple : Antigen 10ng
Blue : Antigen 50ng
Red : Antigen 100ng

Anti-PBK antibody [2C8] used in Flow cytometry (FACS). GTX60560

GTX60560 FACS Image

FACS analysis of HeLa cells using GTX60560 PBK antibody [2C8].
Green : PBK
Red : negative control

Anti-PBK antibody [2C8] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX60560

GTX60560 ICC/IF Image

ICC/IF analysis of HeLa cells using GTX60560 PBK antibody [2C8].
Green : PBK
Blue: DRAQ5 fluorescent DNA dye
Red: Actin filaments

REFERENCE

Application Reference

LH Giang et al. J Exp Clin Cancer Res 2023; 42 (1) : 346 Targeting of RRM2 suppresses DNA damage response and activates apoptosis in atypical teratoid rhabdoid tumor
Application : WB
Reactivity : Human
Submit a Reference

REVIEW

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SDS
Sodium Azide.pdf
Package List Price ($)
$ 399