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PBR antibody, C-term

Anti-PBR antibody, C-term used in IHC (Paraffin sections) (IHC-P). GTX47548
Anti-PBR antibody, C-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX47548
Anti-PBR antibody, C-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX47548
Anti-PBR antibody, C-term used in Western Blot (WB). GTX47548
Anti-PBR antibody, C-term used in Flow cytometry (FACS). GTX47548
Anti-PBR antibody, C-term used in IHC (Paraffin sections) (IHC-P). GTX47548
Anti-PBR antibody, C-term used in IHC (Paraffin sections) (IHC-P). GTX47548
Anti-PBR antibody, C-term used in Western Blot (WB). GTX47548

Cat. No. GTX47548

Host

 Goat

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P, FACS

Reactivity

 Human, Mouse, Rat
Package
100 μg ($399)
See all PBR products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 0.1-0.3μg/ml
ICC/IF 10μg/ml
IHC-P Assay dependent
FACS Assay dependent

Note :

ICC/IF
Strong expression of the protein seen in the nuclear membrane and cytoplasm of NIH3T3 and HeLa cells.
Not tested in other applications.

Calculated MW

19 kDa. ( Note )

Positive Control

The peptide used to generate this antibody is available for purchase (GTX47548-PEP).

PROPERTIES

Form

Liquid

Buffer

TBS, 0.5% BSA

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.50 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Mouse

Immunogen

Peptide with sequence C-RDNSGRRGGSRLPE, from the C Terminus of the protein sequence according to NP_033905.3.

Purification

Purified by ammonium sulphate precipitation followed by antigen affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

Translocator Protein , Bzrp , Ibp , Pbr , Tspo1 , Tspo

Research Area

DATA IMAGES

Anti-PBR antibody, C-term used in IHC (Paraffin sections) (IHC-P). GTX47548

GTX47548 IHC-P Image

IHC-P analysis of human adipose using GTX47548 PBR antibody, C-term.
Antigen retrieval : citrate buffer pH 6
Dilution : 3.75μg/ml

Anti-PBR antibody, C-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX47548

GTX47548 ICC/IF Image

ICC/IF analysis of PFA fixed NIH-3T3 cells using GTX47548 PBR antibody, C-term.
Green : Primary antibody
Blue : DAPI
Negative control : Unimmunized goat IgG
Permeabilization : 0.15% Triton
Dilution : 10μg/ml

Anti-PBR antibody, C-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX47548

GTX47548 ICC/IF Image

ICC/IF analysis of PFA fixed HeLa cells using GTX47548 PBR antibody, C-term.
Green : Primary antibody
Blue : DAPI
Negative control : Unimmunized goat IgG
Permeabilization : 0.15% Triton
Dilution : 10μg/ml

Anti-PBR antibody, C-term used in Western Blot (WB). GTX47548

GTX47548 WB Image

WB analysis of mouse kidney (A) and rat adrenal gland (B) lysate using GTX47548 PBR antibody, C-term.
Dilution : 0.3μg/ml
Loading : 35μg protein in RIPA buffer

Anti-PBR antibody, C-term used in Flow cytometry (FACS). GTX47548

GTX47548 FACS Image

FACS analysis of PFA fixed NIH-3T3 cells using GTX47548 PBR antibody, C-term.
Blue : Primary antibody
Black : Isotype control
Permeabilization : 0.5% Triton
Dilution : 10μg/ml

Anti-PBR antibody, C-term used in IHC (Paraffin sections) (IHC-P). GTX47548

GTX47548 IHC-P Image

IHC-P analysis of human squamous epithelium using GTX47548 PBR antibody, C-term.
Antigen retrieval : citrate buffer Ph 6
Dilution : 3.75μg/ml

Anti-PBR antibody, C-term used in IHC (Paraffin sections) (IHC-P). GTX47548

GTX47548 IHC-P Image

IHC-P analysis of human renal Tubules using GTX47548 PBR antibody, C-term.
Antigen retrieval : citrate buffer pH 6
Dilution : 3.75μg/ml

Anti-PBR antibody, C-term used in Western Blot (WB). GTX47548

GTX47548 WB Image

WB analysis of NIH-3T3 cell lysate using GTX47548 PBR antibody, C-term.
Dilution : 0.1μg/ml
Loading : 35μg protein in RIPA buffer

REFERENCE

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SDS
Sodium Azide.pdf
Package List Price ($)
$ 399