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PBR antibody

Anti-PBR antibody used in Western Blot (WB). GTX130550
Anti-PBR antibody used in Western Blot (WB). GTX130550
Anti-PBR antibody used in Immunoprecipitation (IP). GTX130550

Cat No. GTX130550

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, IP

Reactivity

Human
Package
100 μl ($329),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:10000
IP 1:100-1:500
Not tested in other applications.

Calculated MW

19 kDa. ( Note )

Positive Control

HeLa , U87-MG , A431

PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.574 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human PBR. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

translocator protein , BPBS , BZRP , DBI , IBP , MBR , PBR , PBS , PKBS , PTBR , mDRC , pk18

Background

Present mainly in the mitochondrial compartment of peripheral tissues, the protein encoded by this gene interacts with some benzodiazepines and has different affinities than its endogenous counterpart. The protein is a key factor in the flow of cholesterol into mitochondria to permit the initiation of steroid hormone synthesis. Alternatively spliced transcript variants have been reported; one of the variants lacks an internal exon and is considered non-coding, and the other variants encode the same protein. [provided by RefSeq, Feb 2012]

Database

Research Area

DATA IMAGES

Anti-PBR antibody used in Western Blot (WB). GTX130550

GTX130550 WB Image

Wild-type (WT) and PBR knockout (KO) HeLa cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with PBR antibody (GTX130550) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-PBR antibody used in Western Blot (WB). GTX130550

GTX130550 WB Image

PBR antibody detects PBR protein by western blot analysis. Various whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with PBR antibody (GTX130550) diluted at a dilution of 1:1000.
MCF7 is known to express little to none of PBR. Reference: Cancer Res. 1999 Feb 15;59(4):831-42.

Anti-PBR antibody used in Immunoprecipitation (IP). GTX130550

GTX130550 IP Image

Immunoprecipitation of PBR protein from A431 whole cell extracts using 5 μg of PBR antibody (GTX130550).
Western blot analysis was performed using PBR antibody (GTX130550).
EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.

REFERENCE

There are currently no references for PBR antibody (GTX130550). Be the first to share your publications with this product.

REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 329
$ 169