GeneTex
United States (US)

PBX1 antibody, Internal

GTX88088_2530_WB_18121411_666.jpg
GTX88088_2531_WB_18121411_749.jpg

Cat No. GTX88088

Host Goat
Clonality Polyclonal
Isotype IgG
Application WB
Reactivity Human, Mouse, Rat
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 0.3-1μg/ml
Not tested in other applications.

Calculated MW

47kDa kDa. ( Note )

Positive Control

The peptide used to generate this antibody is available for purchase (GTX88088-PEP).

Predict Reactivity

Dog(>80% identity)
PROPERTIES

Form

Liquid

Buffer

Tris saline pH7.3, 0.02% sodium azide, 0.5% BSA.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.50mg/ml

Antigen Species

Human

Immunogen

Peptide with sequence C-ATNVSAHGSQANSPS, from the internal region of the protein sequence according to NP_002576.1.

Purification

Purified by ammonium sulphate precipitation followed by antigen affinity chromatography

Conjugation

Unconjugated

Note

For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Cellular Localization

Nucleus

Background

This gene encodes a nuclear protein that belongs to the PBX homeobox family of transcriptional factors. Studies in mice suggest that this gene may be involved in the regulation of osteogenesis, and required for skeletal patterning and programming. A chromosomal translocation, t(1;19) involving this gene and TCF3/E2A gene, is associated with pre-B-cell acute lymphoblastic leukemia. The resulting fusion protein, in which the DNA binding domain of E2A is replaced by the DNA binding domain of this protein, transforms cells by constitutively activating transcription of genes regulated by the PBX protein family. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Mar 2011]
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Database

Research Area

DATA IMAGES
GTX88088_2530_WB_18121411_666.jpg

GTX88088 WB Image

WB analysis of K562 lysate using GTX88088 PBX1 antibody, Internal.
Dilution : 0.5µg/ml
Loading : 35µg protein in RIPA buffer

GTX88088_2531_WB_18121411_749.jpg

GTX88088 WB Image

WB analysis of mouse eye lysate using GTX88088 PBX1 antibody, Internal.
Dilution : 0.5µg/ml
Loading : 35µg protein in RIPA buffer