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PBXIP1 antibody

Anti-PBXIP1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX116669
Anti-PBXIP1 antibody used in Western Blot (WB). GTX116669

Cat No. GTX116669

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF

Reactivity

Human
Package
100 μl ($379),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
Not tested in other applications.

Calculated MW

81 kDa. ( Note )

Positive Control

293T , A431 , HeLa

PROPERTIES

Form

Liquid

Buffer

1XPBS (pH7), 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human PBXIP1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2885400

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

PBX homeobox interacting protein 1 , HPIP

Cellular Localization

Cytoplasm , cytoskeleton , Nucleus

Background

Regulator of pre-B-cell leukemia transcription factors (BPXs) function. Inhibits the binding of PBX1-HOX complex to DNA and blocks the transcriptional activity of E2A-PBX1. Tethers estrogen receptor-alpha (ESR1) to microtubules and allows them to influence estrogen receptors-alpha signaling.

Database

Research Area

DATA IMAGES

Anti-PBXIP1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX116669

GTX116669 ICC/IF Image

PBXIP1 antibody detects PBXIP1 protein at cytoplasm and nucleus by immunofluorescent analysis.
Sample: A431 cells were fixed in 4% paraformaldehyde/PBS for 15 min.
Green: PBXIP1 protein stained by PBXIP1 antibody (GTX116669) diluted at 1:500.
Blue: Hoechst 33342 staining.

Anti-PBXIP1 antibody used in Western Blot (WB). GTX116669

GTX116669 WB Image

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with PBXIP1 antibody (GTX116669) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 379
$ 169