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PC1/3 antibody

Anti-PC1/3 antibody used in Western Blot (WB). GTX113797
Anti-PC1/3 antibody used in IHC (Paraffin sections) (IHC-P). GTX113797
Anti-PC1/3 antibody used in IHC (Paraffin sections) (IHC-P). GTX113797

Cat. No. GTX113797

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Human, Mouse
Package
100 μl ($399),
25 μl ($169)
See all PC1/3 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

84 kDa. ( Note )

Positive Control

HCT116

Predict Reactivity

Rat, Zebrafish, Bovine, Pig(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.52 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human PC1/3. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_10618029

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

proprotein convertase subtilisin/kexin type 1 , BMIQ12 , NEC1 , PC1 , PC3 , SPC3

Cellular Localization

Cytoplasmic vesicle , secretory vesicle

Background

The protein encoded by this gene belongs to the subtilisin-like proprotein convertase family. The members of this family are proprotein convertases that process latent precursor proteins into their biologically active products. This encoded protein is a type I proinsulin-processing enzyme that plays a key role in regulating insulin biosynthesis. It is also known to cleave proopiomelanocortin, prorenin, proenkephalin, prodynorphin, prosomatostatin and progastrin. Mutations in this gene are thought to cause obesity. This encoded protein is associated with carcinoid tumors. The use of alternate polyadenylation sites has been found for this gene. Multiple alternatively spliced transcript variants have been described for this gene but their full length nature is not known. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-PC1/3 antibody used in Western Blot (WB). GTX113797

GTX113797 WB Image

Sample (30 μg of whole cell lysate)
A: HCT116
7.5% SDS PAGE
GTX113797 diluted at 1:500
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-PC1/3 antibody used in IHC (Paraffin sections) (IHC-P). GTX113797

GTX113797 IHC-P Image

Immunohistochemical analysis of paraffin-embedded Cal27 xenograft, using PC1/3(GTX113797) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-PC1/3 antibody used in IHC (Paraffin sections) (IHC-P). GTX113797

GTX113797 IHC-P Image

PC1/3 antibody detects PC1/3 protein at cytosol and secreted on mouse intestine by immunohistochemical analysis.
Sample: Paraffin-embedded mouse intestine.
PC1/3 antibody (GTX113797) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

REFERENCE

Application Reference

Knoch KP et al. Mol Metab 2014; 3 (5) : 518-30 ptbp1 is required for glucose-stimulated cap-independent translation of * granule proteins and coxsackieviruses in beta cells.
Application : WB
Reactivity : Mouse
Submit a Reference

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169