PCAF antibody [C3], C-term

Name: PCAF antibody [C3], C-term
Brand: GeneTex
SKU: GTX109666
Price: 399.00 USD
Rating: 3 (1 reviews)

Anti-PCAF antibody [C3], C-term used in Western Blot (WB). GTX109666

Cat. No. GTX109666

Reference 3

Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Application	WB
Reactivity	Human, Mouse
Package	100 μl ($399), 25 μl ($169)

See all PCAF products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Recommended Dilution
WB	1:500-1:3000

Not tested in other applications.

Calculated MW

93 kDa. ( Note )

Positive Control

Mouse brain , 293T , A431 , A375 , HeLa nuclear extract

Predict Reactivity

Zebrafish(>80% identity)

PROPERTIES

Form

Liquid

Buffer

1XPBS (pH7), 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human PCAF. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1950609

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

lysine acetyltransferase 2B , CAF , P/CAF , PCAF

Cellular Localization

Nucleus

Background

CBP and p300 are large nuclear proteins that bind to many sequence-specific factors involved in cell growth and/or differentiation, including c-jun and the adenoviral oncoprotein E1A. The protein encoded by this gene associates with p300/CBP. It has in vitro and in vivo binding activity with CBP and p300, and competes with E1A for binding sites in p300/CBP. It has histone acetyl transferase activity with core histones and nucleosome core particles, indicating that this protein plays a direct role in transcriptional regulation. [provided by RefSeq]

Database

Gene ID: 8850 KAT2B
UniProt: Q92831 KAT2B

Research Area

DATA IMAGES

GTX109666 WB Image

HeLa whole cell and nuclear extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with PCAF antibody [C3], C-term (GTX109666) diluted at 1:1000.

GTX109666 WB Image

Sample (50 ug of whole cell lysate)
A: Mouse brain
7.5% SDS PAGE
GTX109666 diluted at 1:1000

GTX109666 WB Image

PCAF antibody [C3], C-term detects PCAF protein by Western blot analysis.
A. 30 μg 293T whole cell lysate/extract
B. 30 μg A431 whole cell lysate/extract
C. 30 μg A375 whole cell lysate/extract
7.5 % SDS-PAGE
PCAF antibody [C3], C-term (GTX109666) dilution: 1:1000

REFERENCE

Application Reference

Wang SA et al. Sci Rep 2020; 10 (1) : 20870 USP24 stabilizes bromodomain containing proteins to promote lung cancer malignancy.

Application : WB

Reactivity : Human

Sengupta S et al. DNA Repair (Amst) 2018; 66-67 : 1-10 Acetylation of oxidized base repair-initiating NEIL1 DNA glycosylase required for chromatin-bound repair complex formation in the human genome increases cellular resistance to oxidative stress.

Cazzalini O et al. Nucleic Acids Res 2014; CBP and p300 acetylate PCNA to link its degradation with nucleotide excision repair synthesis.

Application : WB

Reactivity : Human

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REVIEW