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PCAF antibody [C3], C-term

Anti-PCAF antibody [C3], C-term used in Western Blot (WB). GTX109666
Anti-PCAF antibody [C3], C-term used in Western Blot (WB). GTX109666
Anti-PCAF antibody [C3], C-term used in Western Blot (WB). GTX109666

Cat. No. GTX109666

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB

Reactivity

Human, Mouse
Package
100 μl ($399),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
Not tested in other applications.

Calculated MW

93 kDa. ( Note )

Positive Control

Mouse brain , 293T , A431 , A375 , HeLa nuclear extract

Predict Reactivity

Zebrafish(>80% identity)

PROPERTIES

Form

Liquid

Buffer

1XPBS (pH7), 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human PCAF. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1950609

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

lysine acetyltransferase 2B , CAF , P/CAF , PCAF

Cellular Localization

Nucleus

Background

CBP and p300 are large nuclear proteins that bind to many sequence-specific factors involved in cell growth and/or differentiation, including c-jun and the adenoviral oncoprotein E1A. The protein encoded by this gene associates with p300/CBP. It has in vitro and in vivo binding activity with CBP and p300, and competes with E1A for binding sites in p300/CBP. It has histone acetyl transferase activity with core histones and nucleosome core particles, indicating that this protein plays a direct role in transcriptional regulation. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-PCAF antibody [C3], C-term used in Western Blot (WB). GTX109666

GTX109666 WB Image

HeLa whole cell and nuclear extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with PCAF antibody [C3], C-term (GTX109666) diluted at 1:1000.

Anti-PCAF antibody [C3], C-term used in Western Blot (WB). GTX109666

GTX109666 WB Image

Sample (50 ug of whole cell lysate)
A: Mouse brain
7.5% SDS PAGE
GTX109666 diluted at 1:1000

Anti-PCAF antibody [C3], C-term used in Western Blot (WB). GTX109666

GTX109666 WB Image

PCAF antibody [C3], C-term detects PCAF protein by Western blot analysis.
A. 30 μg 293T whole cell lysate/extract
B. 30 μg A431 whole cell lysate/extract
C. 30 μg A375 whole cell lysate/extract
7.5 % SDS-PAGE
PCAF antibody [C3], C-term (GTX109666) dilution: 1:1000

REFERENCE

REVIEW

PCAF antibody [C3], C-term Cat. No. GTX109666

Rating ( Average 3 based on 1 users reviews)
Application
Western Blot(WB) ( Average 3 based on 1 users reviews)
Date : Anonymous submitted on 24-Oct-2011
Score :
Application Tested : WB
Sample Species : Ms
Sample : MFB cells
Amount used : 50μg
Blocking : 8% BSA in TBS, 25°C, 1Hr
Primary Antibody : 1:2000, 4°C, 16Hr
SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169