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PCAF antibody

Anti-PCAF antibody used in Western Blot (WB). GTX12188
Anti-PCAF antibody used in Western Blot (WB). GTX12188
Anti-PCAF antibody used in Western Blot (WB). GTX12188
Anti-PCAF antibody used in Immunoprecipitation (IP). GTX12188
Anti-PCAF antibody used in Western Blot (WB). GTX12188

Cat. No. GTX12188

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, IP

Reactivity

Human, Dog
Package
50 μl ($399)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500
IP 5-10 μg
Not tested in other applications.

Calculated MW

93 kDa. ( Note )

Positive Control

Jurkat, MDCK

Product Note

PCAF (p300/CBP associated factor, P/CAF), a type A histone acetyltransferases (HAT), has been identified in several species. Human PCAF is a ubiquitous protein member of the GCN5-related N-acetyltransferase (GNAT) superfamily. It interacts with the p300 and CBP (CREB-binding protein) transcriptional coactivators and may be found in multi-subunit protein complexes formed from more than 20 polypeptides ranging from 10 to 400 kDa.PCAF shows extensive sequence similarity to human and mouse GCN5 HAT. Human PCAF acetylates all four core-histones. It also acetylates nonhistone proteins such as p53, MyoD, TFIIE-β, TFIIE, GATA-1, EKLF, HMGI (Y), and several other cellular and viral proteins. PCAF contributes to transcriptional activation by modifying chromatin and transcriptional factors. It is required in many cell growth and development processes such as myogenesis, and in nuclear receptor mediated or growth factor-signaled cell activation.

PROPERTIES

Form

Liquid

Buffer

0.01M PBS pH7.4

Preservative

15mM Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Mouse

Immunogen

This sequence is identical to mouse PCAF and varies by 2 amino acids residues in chicken. synthetic peptide corresponding to the C-terminal (amino acids 817-832) of human PCAF with N-terminal added cysteine, conjugated to KLH.

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

lysine acetyltransferase 2B , CAF , P/CAF , PCAF

Cellular Localization

Nucleus

Background

CBP and p300 are large nuclear proteins that bind to many sequence-specific factors involved in cell growth and/or differentiation, including c-jun and the adenoviral oncoprotein E1A. The protein encoded by this gene associates with p300/CBP. It has in vitro and in vivo binding activity with CBP and p300, and competes with E1A for binding sites in p300/CBP. It has histone acetyl transferase activity with core histones and nucleosome core particles, indicating that this protein plays a direct role in transcriptional regulation.

Database

Research Area

DATA IMAGES

Anti-PCAF antibody used in Western Blot (WB). GTX12188

GTX12188 WB Image

HeLa whole cell and nuclear extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with PCAF antibody [C3], C-term (GTX12188) diluted at 1:1000.

Anti-PCAF antibody used in Western Blot (WB). GTX12188

GTX12188 WB Image

WB analysis of (Lane 1-2) HeLa, (Lane 3-4) HepG2, (Lane 5-6) Jurkat cell lysate using GTX12188 PCAF antibody at 2 μg/mL.
Lane 1,3,5 : Antibody 2 μg/mL
Lane 2,4,6 : Antibody 2 μg/mL + PCAF immunizing peptide

Anti-PCAF antibody used in Western Blot (WB). GTX12188

GTX12188 WB Image

WB analysis of human PCAF recombinant protein using GTX12188 PCAF antibody.
Lane 1 : 2 μg/mL
Lane 2 : Negative control (without antibody)

Anti-PCAF antibody used in Immunoprecipitation (IP). GTX12188

GTX12188 IP Image

IP analysis of Jurkat cell lysate using GTX12188 PCAF antibody.
Lane 1 : 5 μg/mL antibody
Lane 2 : Negative control: without antibody
Lane 3 : 10 μg/mL antibody
Lane 4 : Negative control: without cell extract

Anti-PCAF antibody used in Western Blot (WB). GTX12188

GTX12188 WB Image

WB analysis of Y79 cell lysate using GTX12188 PCAF antibody.
Lane 1 : 2 μg/mL
Lane 2 : Antibody 2 μg/mL + PCAF immunizing peptide (human, 817-832)

REFERENCE

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REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399