GeneTex
United States (US)

PCBP1 antibody [AT2A10]

GTX53759_WB_18121410_582.jpg

Cat No. GTX53759

Host Mouse
Clonality Monoclonal
Clone Name AT2A10
Isotype IgG2a
Application WB, ELISA
Reactivity Human
APPLICATION

Application Note

The antibody has been tested by ELISA, Western blot analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results. Recommended starting dilution is 1:500.

Calculated MW

37 kDa. ( Note )
PROPERTIES

Form

Liquid

Buffer

Phosphate-Buffered Saline (pH 7.4) containing 10% Glycerol, 0.02% Sodium Azide.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1mg/ml(Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant human PCBP1 (1-163aa) purified from E. coli

Purification

By protein-A affinity chromatography

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

poly(rC) binding protein 1 Antibody , hnRNP-E1 Antibody , HNRPE1 Antibody , hnRNP-X Antibody , HNRPX Antibody

Background

This intronless gene is thought to have been generated by retrotransposition of a fully processed PCBP-2 mRNA. This gene and PCBP-2 have paralogues (PCBP3 and PCBP4) which are thought to have arisen as a result of duplication events of entire genes. The protein encoded by this gene appears to be multifunctional. It along with PCBP-2 and hnRNPK corresponds to the major cellular poly(rC)-binding protein. It contains three K-homologous (KH) domains which may be involved in RNA binding. This encoded protein together with PCBP-2 also functions as translational coactivators of poliovirus RNA via a sequence-specific interaction with stem-loop IV of the IRES and promote poliovirus RNA replication by binding to its 5'-terminal cloverleaf structure. It has also been implicated in translational control of the 15-lipoxygenase mRNA, human Papillomavirus type 16 L2 mRNA, and hepatitis A virus RNA. The encoded protein is also suggested to play a part in formation of a sequence-specific alpha-globin m

Database

DATA IMAGES
GTX53759_WB_18121410_582.jpg

GTX53759 WB Image

WB analysis of HeLa and HepG2 lysate s(40ug per lane) using PCBP1 antibody at a dilution of 1:500.

Package List Price ($)
$ 319