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PCBP1 antibody

Anti-PCBP1 antibody used in Western Blot (WB). GTX64891
Anti-PCBP1 antibody used in IHC (Paraffin sections) (IHC-P). GTX64891
Anti-PCBP1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX64891
Anti-PCBP1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX64891
Anti-PCBP1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX64891

Cat. No. GTX64891

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399)
See all PCBP1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500 - 1:2000
ICC/IF 1:50 - 1:200
IHC-P 1:50 - 1:100
Not tested in other applications.

Calculated MW

37 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 100-200 of human PCBP1 (NP_006187.2).

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

poly(rC) binding protein 1 , HEL-S-85 , HNRPE1 , HNRPX , hnRNP-E1 , hnRNP-X

Cellular Localization

Nucleus,Cytoplasm

Background

This intronless gene is thought to have been generated by retrotransposition of a fully processed PCBP-2 mRNA. This gene and PCBP-2 have paralogues (PCBP3 and PCBP4) which are thought to have arisen as a result of duplication events of entire genes. The protein encoded by this gene appears to be multifunctional. It along with PCBP-2 and hnRNPK corresponds to the major cellular poly(rC)-binding protein. It contains three K-homologous (KH) domains which may be involved in RNA binding. This encoded protein together with PCBP-2 also functions as translational coactivators of poliovirus RNA via a sequence-specific interaction with stem-loop IV of the IRES and promote poliovirus RNA replication by binding to its 5'-terminal cloverleaf structure. It has also been implicated in translational control of the 15-lipoxygenase mRNA, human Papillomavirus type 16 L2 mRNA, and hepatitis A virus RNA. The encoded protein is also suggested to play a part in formation of a sequence-specific alpha-globin mRNP complex which is associated with alpha-globin mRNA stability. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-PCBP1 antibody used in Western Blot (WB). GTX64891

GTX64891 WB Image

WB analysis of various sample lysates using GTX64891 PCBP1 antibody.
Dilution : 1:1000
Loading : 25μg per lane

Anti-PCBP1 antibody used in IHC (Paraffin sections) (IHC-P). GTX64891

GTX64891 IHC-P Image

IHC-P analysis of human stomach tissue using GTX64891 PCBP1 antibody.
Dilution : 1:100

Anti-PCBP1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX64891

GTX64891 ICC/IF Image

ICC/IF analysis of C6 cells using GTX64891 PCBP1 antibody.
Blue : DAPI
Dilution : 1:100

Anti-PCBP1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX64891

GTX64891 ICC/IF Image

ICC/IF analysis of U2OS cells using GTX64891 PCBP1 antibody.
Blue : DAPI
Dilution : 1:100

Anti-PCBP1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX64891

GTX64891 ICC/IF Image

ICC/IF analysis of NIH/3T3 cells using GTX64891 PCBP1 antibody.
Blue : DAPI
Dilution : 1:100

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399