*Optimal dilutions/concentrations should be determined by the researcher.
Not tested in other applications.
Jurkat , Raji , K562 , NCI-H929
Rat, Bovine, Pig(>80% identity)
1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
1.03 mg/ml (Please refer to the vial label for the specific concentration.)
Recombinant protein encompassing a sequence within the center region of human PCBP2. The exact sequence is proprietary.
Purified by antigen-affinity chromatography.
For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Poly(Rc) Binding Protein 2,Hnrnpe2,Hnrpe2,Hnrnp-E2,Pcbp2
Nucleus , Cytoplasm
The protein encoded by this gene appears to be multifunctional. Along with PCBP-1 and hnRNPK, it is one of the major cellular poly(rC)-binding proteins. The encoded protein contains three K-homologous (KH) domains which may be involved in RNA binding. Together with PCBP-1, this protein also functions as a translational coactivator of poliovirus RNA via a sequence-specific interaction with stem-loop IV of the IRES, promoting poliovirus RNA replication by binding to its 5'-terminal cloverleaf structure. It has also been implicated in translational control of the 15-lipoxygenase mRNA, human papillomavirus type 16 L2 mRNA, and hepatitis A virus RNA. The encoded protein is also suggested to play a part in formation of a sequence-specific alpha-globin mRNP complex which is associated with alpha-globin mRNA stability. This multiexon structural mRNA is thought to be retrotransposed to generate PCBP-1, an intronless gene with functions similar to that of PCBP2. This gene and PCBP-1 have paralogous genes (PCBP3 and PCBP4) which are thought to have arisen as a result of duplication events of entire genes. Thsi gene also has two processed pseudogenes (PCBP2P1 and PCBP2P2). Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]
GTX114617 IHC-P Image
Immunohistochemical analysis of paraffin-embedded human muscle, using PCBP2(GTX114617) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
GTX114617 WB Image
PCBP2 antibody detects PCBP2 protein by western blot analysis. Various whole cell extracts (30 μg) were separated by 12 % SDS-PAGE, and blotted with PCBP2 antibody (GTX114617) diluted by 1:1000
GTX114617 ICC/IF Image
PCBP2 antibody detects PCBP2 protein at nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: PCBP2 protein stained by PCBP2 antibody (GTX114617) diluted at 1:750.
Red: phalloidin, a cytoskeleton marker, diluted at 1:200.
Scale bar = 10 μm.
GTX114617 IHC-P Image
PCBP2 antibody detects PCBP2 protein at cytoplasm and nucleus in mouse brain by immunohistochemical analysis.
Sample: Paraffin-embedded mouse brain.
PCBP2 antibody (GTX114617) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
GTX114617 IP Image
Immunoprecipitation of PCBP2 protein from Jurkat whole cell extracts using 5 μg of PCBP2 antibody (GTX114617).
Western blot analysis was performed using PCBP2 antibody (GTX114617).
EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.