GeneTex
  • Country / Location Selection

United States (US)

PCBP2 antibody

Anti-PCBP2 antibody used in IHC (Paraffin sections) (IHC-P). GTX114617
Anti-PCBP2 antibody used in Western Blot (WB). GTX114617
Anti-PCBP2 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX114617
Anti-PCBP2 antibody used in IHC (Paraffin sections) (IHC-P). GTX114617
Anti-PCBP2 antibody used in Immunoprecipitation (IP). GTX114617

Cat No. GTX114617

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P, IP
Reactivity Human, Mouse
Package
100 μl ($319),
25 μl ($169)
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IP 1:100-1:500
Not tested in other applications.

Calculated MW

39 kDa. ( Note )

Positive Control

Jurkat , Raji , K562 , NCI-H929

Predict Reactivity

Rat, Bovine, Pig(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.03 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human PCBP2. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Poly(Rc) Binding Protein 2,Hnrnpe2,Hnrpe2,Hnrnp-E2,Pcbp2

Cellular Localization

Nucleus , Cytoplasm

Background

The protein encoded by this gene appears to be multifunctional. Along with PCBP-1 and hnRNPK, it is one of the major cellular poly(rC)-binding proteins. The encoded protein contains three K-homologous (KH) domains which may be involved in RNA binding. Together with PCBP-1, this protein also functions as a translational coactivator of poliovirus RNA via a sequence-specific interaction with stem-loop IV of the IRES, promoting poliovirus RNA replication by binding to its 5'-terminal cloverleaf structure. It has also been implicated in translational control of the 15-lipoxygenase mRNA, human papillomavirus type 16 L2 mRNA, and hepatitis A virus RNA. The encoded protein is also suggested to play a part in formation of a sequence-specific alpha-globin mRNP complex which is associated with alpha-globin mRNA stability. This multiexon structural mRNA is thought to be retrotransposed to generate PCBP-1, an intronless gene with functions similar to that of PCBP2. This gene and PCBP-1 have paralogous genes (PCBP3 and PCBP4) which are thought to have arisen as a result of duplication events of entire genes. Thsi gene also has two processed pseudogenes (PCBP2P1 and PCBP2P2). Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]

Database

DATA IMAGES
Anti-PCBP2 antibody used in IHC (Paraffin sections) (IHC-P). GTX114617

GTX114617 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human muscle, using PCBP2(GTX114617) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-PCBP2 antibody used in Western Blot (WB). GTX114617

GTX114617 WB Image

PCBP2 antibody detects PCBP2 protein by western blot analysis. Various whole cell extracts (30 μg) were separated by 12 % SDS-PAGE, and blotted with PCBP2 antibody (GTX114617) diluted by 1:1000

Anti-PCBP2 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX114617

GTX114617 ICC/IF Image

PCBP2 antibody detects PCBP2 protein at nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: PCBP2 protein stained by PCBP2 antibody (GTX114617) diluted at 1:750.
Red: phalloidin, a cytoskeleton marker, diluted at 1:200.
Scale bar = 10 μm.

Anti-PCBP2 antibody used in IHC (Paraffin sections) (IHC-P). GTX114617

GTX114617 IHC-P Image

PCBP2 antibody detects PCBP2 protein at cytoplasm and nucleus in mouse brain by immunohistochemical analysis.
Sample: Paraffin-embedded mouse brain.
PCBP2 antibody (GTX114617) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-PCBP2 antibody used in Immunoprecipitation (IP). GTX114617

GTX114617 IP Image

Immunoprecipitation of PCBP2 protein from Jurkat whole cell extracts using 5 μg of PCBP2 antibody (GTX114617).
Western blot analysis was performed using PCBP2 antibody (GTX114617).
EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.

REFERENCE
There are currently no references for PCBP2 antibody (GTX114617). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for PCBP2 antibody (GTX114617). Be the first to share your experience with this product.
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 319
$ 169