APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1-3μg/ml |
Not tested in other applications.
Calculated MW
Positive Control
The peptide used to generate this antibody is available for purchase (GTX88982-PEP).
Product Note
This antibody is expected to recognise all reported isoforms (NP_065151.1, NP_127501.1, NP_127502.1 and NP_127503.1).
Predict Reactivity
Mouse, Rat, Dog(>80% identity)
PROPERTIES
Form
Liquid
Buffer
TBS, 0.5% BSA
Preservative
0.02% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
0.50 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Peptide with sequence C-NGSKKAERQKFSPY, from the C Terminus of the protein sequence according to NP_065151.1; NP_127501.1; NP_127502.1; NP_127503.1 .
Purification
Purified by ammonium sulphate precipitation followed by antigen affinity chromatography
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
poly(rC) binding protein 4 , CBP , LIP4 , MCG10
Cellular Localization
Cytoplasm
Background
This gene encodes a member of the KH-domain protein subfamily. Proteins of this subfamily, also referred to as alpha-CPs, bind to RNA with a specificity for C-rich pyrimidine regions. Alpha-CPs play important roles in post-transcriptional activities and have different cellular distributions. This gene is induced by the p53 tumor suppressor, and the encoded protein can suppress cell proliferation by inducing apoptosis and cell cycle arrest in G(2)-M. This gene's protein is found in the cytoplasm, yet it lacks the nuclear localization signals found in other subfamily members. Multiple alternatively spliced transcript variants have been described, but the full-length nature for only some has been determined. [provided by RefSeq, Jul 2008]
Database
Research Area
DATA IMAGES
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GTX88982 WB Image
WB analysis of HeLa cell lysate using GTX88982 PCBP4 antibody, C-term.
Dilution : 0.03μg/ml
Loading : 35μg protein in RIPA buffer
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GTX88982 WB Image
WB analysis of HEK293 overexpressing PCBP4 (mock transfection in first lane) using GTX88982 PCBP4 antibody, C-term.
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REFERENCE
There are currently no references for PCBP4 antibody, C-term (GTX88982). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for PCBP4 antibody, C-term (GTX88982). Be the first to share your experience with this product.
