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PCCA antibody, N-term

Anti-PCCA antibody, N-term used in IHC (Paraffin sections) (IHC-P). GTX47779
Anti-PCCA antibody, N-term used in Western Blot (WB). GTX47779

Cat No. GTX47779

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, IHC-P

Reactivity

Human
Package
50 μg ($329)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 0.2-2.5 ug/ml
IHC-P 2-10 ug/ml
Not tested in other applications.

Calculated MW

80 kDa. ( Note )

Positive Control

Transfected 293T

PROPERTIES

Form

Liquid

Buffer

1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.5-1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

A synthetic peptide corresponding to a N-terminal region of Human PCCA

Purification

Affinity Purified

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

propionyl-CoA carboxylase subunit alpha

Cellular Localization

Mitochondrion matrix

Background

The protein encoded by this gene is the alpha subunit of the heterodimeric mitochondrial enzyme Propionyl-CoA carboxylase. PCCA encodes the biotin-binding region of this enzyme. Mutations in either PCCA or PCCB (encoding the beta subunit) lead to an enzyme deficiency resulting in propionic acidemia. Multiple transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, May 2010]

Database

Research Area

DATA IMAGES

Anti-PCCA antibody, N-term used in IHC (Paraffin sections) (IHC-P). GTX47779

GTX47779 IHC-P Image

IHC-P analysis of human heart tissue using GTX47779 PCCA antibody at 1:600. Left to right:DAPI, PCCA, Merge. Heat-induced antigen retrieval method utilizing Sodium Citrate buffer was performed.

Anti-PCCA antibody, N-term used in Western Blot (WB). GTX47779

GTX47779 WB Image

WB analysis of tranfected 293T cells using GTX47779 PCCA antibody at 0.2-1μg/ml.

REFERENCE

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REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf