GeneTex
United States (US)

PCCB antibody [N2C3]

GTX105204_39897_IFA_19011506_623.jpg
GTX105204_39897_20150116_IHC_M_19011506_599.jpg
GTX105204_IHC_19011506_573.jpg
GTX105204_WB_19011506_413.jpg

Cat No. GTX105204

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P
Reactivity Human, Mouse
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

58 kDa. ( Note )

Positive Control

HepG2

Predict Reactivity

Rat, Zebrafish, Bovine, Pig, Xenopus laevis, Rhesus Monkey(>80% identity)
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.54mg/ml(Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human PCCB. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

DKFZp451E113 antibody, PCCB antibody, propanoyl-CoA:carbon dioxide ligase subunit beta antibody, "propionyl Coenzyme A carboxylase, beta polypeptide antibody", PCCase subunit beta antibody, "propionyl-CoA carboxylase beta chain, mitochondrial antibody", "propionyl CoA carboxylase, beta polypeptide antibody"

Cellular Localization

Mitochondrion matrix

Background

The protein encoded by this gene is a subunit of the propionyl-CoA carboxylase (PCC) enzyme, which is involved in the catabolism of propionyl-CoA. PCC is a mitochondrial enzyme that probably acts as a dodecamer of six alpha subunits and six beta subunits. This gene encodes the beta subunit of PCC. Defects in this gene are a cause of propionic acidemia type II (PA-2). [provided by RefSeq]

Database

Research Area

DATA IMAGES
GTX105204_39897_IFA_19011506_623.jpg

GTX105204 ICC/IF Image

Immunofluorescence analysis of methanol-fixed Hep G2, using PCCB(GTX105204) antibody at 1:500 dilution.

GTX105204_39897_20150116_IHC_M_19011506_599.jpg

GTX105204 IHC-P Image

PCCB antibody [N2C3] detects PCCB protein at mitochondria on mouse kidney by immunohistochemical analysis.
Sample: Paraffin-embedded mouse kidney.
PCCB antibody [N2C3] (GTX105204) diluted at 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX105204_IHC_19011506_573.jpg

GTX105204 IHC-P Image

Immunohistochemical analysis of paraffin-embedded Lung Ad Cancer PC14 xenograft, using PCCB(GTX105204 ) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX105204_WB_19011506_413.jpg

GTX105204 WB Image

Sample (30 µg whole cell lysate)
A: HepG2 (GTX27900)
7.5% SDS PAGE
GTX105204 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169