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PCCB antibody [N2C3]

Anti-PCCB antibody [N2C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX105204
Anti-PCCB antibody [N2C3] used in IHC (Paraffin sections) (IHC-P). GTX105204
Anti-PCCB antibody [N2C3] used in IHC (Paraffin sections) (IHC-P). GTX105204
Anti-PCCB antibody [N2C3] used in Western Blot (WB). GTX105204

Cat No. GTX105204

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P
Reactivity Human, Mouse
Package
100 μl ($319),
25 μl ($169)
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

58 kDa. ( Note )

Positive Control

HepG2

Predict Reactivity

Rat, Zebrafish, Bovine, Pig, Xenopus laevis, Rhesus Monkey(>80% identity)
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.54 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human PCCB. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

propionyl-CoA carboxylase subunit beta

Cellular Localization

Mitochondrion matrix

Background

The protein encoded by this gene is a subunit of the propionyl-CoA carboxylase (PCC) enzyme, which is involved in the catabolism of propionyl-CoA. PCC is a mitochondrial enzyme that probably acts as a dodecamer of six alpha subunits and six beta subunits. This gene encodes the beta subunit of PCC. Defects in this gene are a cause of propionic acidemia type II (PA-2). [provided by RefSeq]

Database

Research Area

DATA IMAGES
Anti-PCCB antibody [N2C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX105204

GTX105204 ICC/IF Image

Immunofluorescence analysis of methanol-fixed Hep G2, using PCCB(GTX105204) antibody at 1:500 dilution.

Anti-PCCB antibody [N2C3] used in IHC (Paraffin sections) (IHC-P). GTX105204

GTX105204 IHC-P Image

PCCB antibody [N2C3] detects PCCB protein at mitochondria on mouse kidney by immunohistochemical analysis.
Sample: Paraffin-embedded mouse kidney.
PCCB antibody [N2C3] (GTX105204) diluted at 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-PCCB antibody [N2C3] used in IHC (Paraffin sections) (IHC-P). GTX105204

GTX105204 IHC-P Image

Immunohistochemical analysis of paraffin-embedded Lung Ad Cancer PC14 xenograft, using PCCB(GTX105204 ) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-PCCB antibody [N2C3] used in Western Blot (WB). GTX105204

GTX105204 WB Image

Sample (30 µg whole cell lysate)
A: HepG2 (GTX27900)
7.5% SDS PAGE
GTX105204 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE
There are currently no references for PCCB antibody [N2C3] (GTX105204). Be the first to share your publications with this product.
REVIEW
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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169