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PCCB antibody [N2C3]

Anti-PCCB antibody [N2C3] used in Western Blot (WB). GTX105204
Anti-PCCB antibody [N2C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX105204
Anti-PCCB antibody [N2C3] used in IHC (Paraffin sections) (IHC-P). GTX105204
Anti-PCCB antibody [N2C3] used in IHC (Paraffin sections) (IHC-P). GTX105204

Cat. No. GTX105204

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse
Package
100 μl ($399),
25 μl ($169)
See all PCCB products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

58 kDa. ( Note )

Positive Control

HepG2

Predict Reactivity

Rat, Zebrafish, Bovine, Pig, Xenopus laevis, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris-Glycine (pH7), 10% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.54 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human PCCB. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1951127

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

propionyl-CoA carboxylase subunit beta

Cellular Localization

Mitochondrion matrix

Background

The protein encoded by this gene is a subunit of the propionyl-CoA carboxylase (PCC) enzyme, which is involved in the catabolism of propionyl-CoA. PCC is a mitochondrial enzyme that probably acts as a dodecamer of six alpha subunits and six beta subunits. This gene encodes the beta subunit of PCC. Defects in this gene are a cause of propionic acidemia type II (PA-2). [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-PCCB antibody [N2C3] used in Western Blot (WB). GTX105204

GTX105204 WB Image

Sample (30 μg whole cell lysate)
A: HepG2 (GTX27900)
7.5% SDS PAGE
GTX105204 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-PCCB antibody [N2C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX105204

GTX105204 ICC/IF Image

Immunofluorescence analysis of methanol-fixed Hep G2, using PCCB(GTX105204) antibody at 1:500 dilution.

Anti-PCCB antibody [N2C3] used in IHC (Paraffin sections) (IHC-P). GTX105204

GTX105204 IHC-P Image

Immunohistochemical analysis of paraffin-embedded Lung Ad Cancer PC14 xenograft, using PCCB(GTX105204 ) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-PCCB antibody [N2C3] used in IHC (Paraffin sections) (IHC-P). GTX105204

GTX105204 IHC-P Image

PCCB antibody [N2C3] detects PCCB protein at mitochondria on mouse kidney by immunohistochemical analysis.
Sample: Paraffin-embedded mouse kidney.
PCCB antibody [N2C3] (GTX105204) diluted at 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

REFERENCE

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REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169