Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with PCDH1 antibody [C2C3], C-term (GTX114620) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

Immunohistochemical analysis of paraffin-embedded BT474 xenograft, using PCDH1(GTX114620) antibody at 1:100 dilution.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min