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PCDH1 antibody [C2C3], C-term

Orthogonal
Anti-PCDH1 antibody [C2C3], C-term used in IHC (Paraffin sections) (IHC-P). GTX114620
Anti-PCDH1 antibody [C2C3], C-term used in Western Blot (WB). GTX114620

Cat. No. GTX114620

Reference 2

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Human, Mouse
Package
100 μl ($399),
25 μl ($169)
See all PCDH1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

115 kDa. ( Note )

Positive Control

HCT116

Predict Reactivity

Bovine(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.69 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the C-terminus region of human PCDH1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_10620484

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

protocadherin 1 , PC42 , PCDH42

Background

This gene belongs to the protocadherin subfamily within the cadherin superfamily. The encoded protein is a membrane protein found at cell-cell boundaries. It is involved in neural cell adhesion, suggesting a possible role in neuronal development. The protein includes an extracelllular region, containing 7 cadherin-like domains, a transmembrane region and a C-terminal cytoplasmic region. Cells expressing the protein showed cell aggregation activity. Alternative splicing occurs in this gene. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-PCDH1 antibody [C2C3], C-term used in IHC (Paraffin sections) (IHC-P). GTX114620

GTX114620 IHC-P Image

Immunohistochemical analysis of paraffin-embedded BT474 xenograft, using PCDH1(GTX114620) antibody at 1:100 dilution.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-PCDH1 antibody [C2C3], C-term used in Western Blot (WB). GTX114620

GTX114620 WB Image

Orthogonal

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with PCDH1 antibody [C2C3], C-term (GTX114620) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

REFERENCE

Application Reference

X Du et al. BMC Cancer 2023; 23 (1) : 1102 PCDH1, a poor prognostic biomarker and potential target for pancreatic adenocarcinoma metastatic therapy
Application : WB, IHC-P
Butko MT et al. Proc Natl Acad Sci U S A 2013; 110 (8) : E726-35 In vivo quantitative proteomics of somatosensory cortical synapses shows which protein levels are modulated by sensory deprivation.
Application : WB
Reactivity : Mouse
Submit a Reference

REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169