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PCK2 antibody [C1C2], Internal

Anti-PCK2 antibody [C1C2], Internal used in Western Blot (WB). GTX104663
Anti-PCK2 antibody [C1C2], Internal used in IHC (Paraffin sections) (IHC-P). GTX104663
Anti-PCK2 antibody [C1C2], Internal used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX104663
Anti-PCK2 antibody [C1C2], Internal used in Western Blot (WB). GTX104663
Anti-PCK2 antibody [C1C2], Internal used in Western Blot (WB). GTX104663

Cat No. GTX104663

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P
Reactivity Human, Mouse, Rat
Package
100 μl ($319),
25 μl ($169)
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

71 kDa. ( Note )

Positive Control

HepG2 , Neuro2A , GL261 , PC-12

Predict Reactivity

Bovine, Pig, Rhesus Monkey(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 40% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the center region of human PCK2. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

phosphoenolpyruvate carboxykinase 2, mitochondrial , PEPCK , PEPCK-M , PEPCK2

Cellular Localization

Mitochondrion

Background

This gene encodes a member of the phosphoenolpyruvate carboxykinase (GTP) family. The protein is a mitochondrial enzyme that catalyzes the conversion of oxaloacetate to phosphoenolpyruvate in the presence of GTP. A cytosolic form encoded by a different gene has also been characterized and is the key enzyme of gluconeogenesis in the liver. The encoded protein may serve a similar function, although it is constitutively expressed and not modulated by hormones such as glucagon and insulin that regulate the cytosolic form. Alternatively spliced transcript variants have been described. [provided by RefSeq]

Database

Research Area

DATA IMAGES
Anti-PCK2 antibody [C1C2], Internal used in Western Blot (WB). GTX104663

GTX104663 WB Image

PCK2 antibody [C1C2], Internal detects PCK2 protein by Western blot analysis.
A. 30 µg Neuro2A whole cell lysate/extract
B. 30 µg GL261 whole cell lysate/extract
7.5 % SDS-PAGE
PCK2 antibody [C1C2], Internal (GTX104663) dilution: 1:1000

Anti-PCK2 antibody [C1C2], Internal used in IHC (Paraffin sections) (IHC-P). GTX104663

GTX104663 IHC-P Image

Immunohistochemical analysis of paraffin-embedded OVCAR3 xenograft, using PCK2(GTX104663) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-PCK2 antibody [C1C2], Internal used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX104663

GTX104663 ICC/IF Image

PCK2 antibody [C1C2], Internal detects PCK2 protein at mitochondria by immunofluorescent analysis.
Sample: Hep G2 cells were fixed in 2% paraformaldehyde/culture medium at 37oC for 30 min.
Green: PCK2 protein stained by PCK2 antibody [C1C2], Internal (GTX104663) diluted at 1:500.
Blue: Hoechst 33342 staining.
Scale bar = 10 μm.

Anti-PCK2 antibody [C1C2], Internal used in Western Blot (WB). GTX104663

GTX104663 WB Image

PCK2 antibody [C1C2], Internal detects PCK2 protein by Western blot analysis.
A. 30 µg HepG2 whole cell lysate/extract
7.5 % SDS-PAGE
PCK2 antibody [C1C2], Internal (GTX104663) dilution: 1:1000

Anti-PCK2 antibody [C1C2], Internal used in Western Blot (WB). GTX104663

GTX104663 WB Image

PCK2 antibody [C1C2], Internal detects PCK2 protein by Western blot analysis.
A. 30 µg PC-12 whole cell lysate/extract
7.5 % SDS-PAGE
PCK2 antibody [C1C2], Internal (GTX104663) dilution: 1:1000

REFERENCE
REVIEW
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SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169