PCK2 antibody [C1C2], Internal

Name: PCK2 antibody [C1C2], Internal
Brand: GeneTex
SKU: GTX104663
Price: 399.00 USD
Rating: 5 (2 reviews)

Anti-PCK2 antibody [C1C2], Internal used in Western Blot (WB). GTX104663

Anti-PCK2 antibody [C1C2], Internal used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX104663

Anti-PCK2 antibody [C1C2], Internal used in IHC (Paraffin sections) (IHC-P). GTX104663

Cat. No. GTX104663

Reference 2

Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Application	WB, ICC/IF, IHC-P
Reactivity	Human, Mouse, Rat
Package	100 μl ($399), 25 μl ($169)

See all PCK2 products

View product citations for antibody GTX104663 on CiteAb

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Recommended Dilution
WB	1:500-1:3000
ICC/IF	1:100-1:1000
IHC-P	1:100-1:1000

Not tested in other applications.

Calculated MW

71 kDa. ( Note )

Positive Control

HepG2 , Neuro2A , GL261 , PC-12

Predict Reactivity

Bovine, Pig, Xenopus tropicalis, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 40% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the center region of human PCK2. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1241162

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

phosphoenolpyruvate carboxykinase 2, mitochondrial , PEPCK , PEPCK-M , PEPCK2

Cellular Localization

Mitochondrion

Background

This gene encodes a member of the phosphoenolpyruvate carboxykinase (GTP) family. The protein is a mitochondrial enzyme that catalyzes the conversion of oxaloacetate to phosphoenolpyruvate in the presence of GTP. A cytosolic form encoded by a different gene has also been characterized and is the key enzyme of gluconeogenesis in the liver. The encoded protein may serve a similar function, although it is constitutively expressed and not modulated by hormones such as glucagon and insulin that regulate the cytosolic form. Alternatively spliced transcript variants have been described. [provided by RefSeq]

Database

Gene ID: 5106 PCK2
UniProt: Q16822 PCK2

Research Area

DATA IMAGES

GTX104663 WB Image

PCK2 antibody [C1C2], Internal detects PCK2 protein by Western blot analysis.
A. 30 μg Neuro2A whole cell lysate/extract
B. 30 μg GL261 whole cell lysate/extract
7.5 % SDS-PAGE
PCK2 antibody [C1C2], Internal (GTX104663) dilution: 1:1000

GTX104663 ICC/IF Image

PCK2 antibody [C1C2], Internal detects PCK2 protein at mitochondria by immunofluorescent analysis.
Sample: Hep G2 cells were fixed in 2% paraformaldehyde/culture medium at 37oC for 30 min.
Green: PCK2 protein stained by PCK2 antibody [C1C2], Internal (GTX104663) diluted at 1:500.
Blue: Hoechst 33342 staining.
Scale bar = 10 μm.

GTX104663 IHC-P Image

Immunohistochemical analysis of paraffin-embedded OVCAR3 xenograft, using PCK2(GTX104663) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX104663 WB Image

PCK2 antibody [C1C2], Internal detects PCK2 protein by Western blot analysis.
A. 30 μg PC-12 whole cell lysate/extract
7.5 % SDS-PAGE
PCK2 antibody [C1C2], Internal (GTX104663) dilution: 1:1000

GTX104663 WB Image

PCK2 antibody [C1C2], Internal detects PCK2 protein by Western blot analysis.
A. 30 μg HepG2 whole cell lysate/extract
7.5 % SDS-PAGE
PCK2 antibody [C1C2], Internal (GTX104663) dilution: 1:1000

GTX104663 WB Image

The data was published in the journal BMC Genomics in 2015. PMID: 25652794

REFERENCE

Application Reference

Lin LL et al. BMC Genomics 2015; 16 : 41 Integrating transcriptomics and proteomics to show that tanshinone IIA suppresses cell growth by blocking glucose metabolism in gastric cancer cells.

Application : WB

Reactivity : Human

Wu YH et al. PLoS One 2013; 8 (8) : e70642 Quantitative proteomic analysis of human lung tumor xenografts treated with the ectopic ATP synthase inhibitor citreoviridin.

Application : WB

Reactivity : Human

Submit a Reference

REVIEW