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PCK2 antibody, Internal

Anti-PCK2 antibody, Internal used in Western Blot (WB). GTX89464

Cat. No. GTX89464

Host

 Goat

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF

Reactivity

 Human, Mouse, Rat, Guinea pig
Package
100 μg ($399)
See all PCK2 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 2-4μg/ml
ICC/IF Assay dependent
Not tested in other applications.

Calculated MW

71 kDa. ( Note )

Positive Control

The peptide used to generate this antibody is available for purchase (GTX89464-PEP).

Product Note

This antibody is expected to recognise isoform 1 (NP_004554) only, no cross-reactivity with PCK1.

Predict Reactivity

Bovine, Dog(>80% identity)

PROPERTIES

Form

Liquid

Buffer

TBS, 0.5% BSA

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.50 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Peptide with sequence KPWKPGDKEPCAH, from the internal region of the protein sequence according to NP_004554.2.

Purification

Purified by ammonium sulphate precipitation followed by antigen affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

phosphoenolpyruvate carboxykinase 2, mitochondrial , PEPCK , PEPCK-M , PEPCK2

Cellular Localization

Mitochondrion

Background

This gene encodes a mitochondrial enzyme that catalyzes the conversion of oxaloacetate to phosphoenolpyruvate in the presence of guanosine triphosphate (GTP). A cytosolic form of this protein is encoded by a different gene and is the key enzyme of gluconeogenesis in the liver. Alternatively spliced transcript variants have been described. [provided by RefSeq, Apr 2014]

Database

Research Area

DATA IMAGES

Anti-PCK2 antibody, Internal used in Western Blot (WB). GTX89464

GTX89464 WB Image

WB analysis of human liver lysate using GTX89464 PCK2 antibody, Internal.
Dilution : 2μg/ml
Loading : 35μg protein in RIPA buffer

REFERENCE

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REVIEW

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SDS
Sodium Azide.pdf
Package List Price ($)
$ 399