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PCK2 antibody

KO/KD
Anti-PCK2 antibody used in Western Blot (WB). GTX114919
Anti-PCK2 antibody used in Western Blot (WB). GTX114919
Anti-PCK2 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX114919
Anti-PCK2 antibody used in Western Blot (WB). GTX114919
Anti-PCK2 antibody used in IHC (Paraffin sections) (IHC-P). GTX114919
Anti-PCK2 antibody used in Western Blot (WB). GTX114919

Cat. No. GTX114919

Reference 2

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)
See all PCK2 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

71 kDa. ( Note )

Positive Control

Neuro2A , GL261 , PC-12 , 293T , HeLa , HepG2

Predict Reactivity

Bovine, Pig, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the N-terminus region of human PCK2. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_10621116

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

phosphoenolpyruvate carboxykinase 2, mitochondrial , PEPCK , PEPCK-M , PEPCK2

Cellular Localization

Mitochondrion

Background

This gene encodes a member of the phosphoenolpyruvate carboxykinase (GTP) family. The protein is a mitochondrial enzyme that catalyzes the conversion of oxaloacetate to phosphoenolpyruvate in the presence of GTP. A cytosolic form encoded by a different gene has also been characterized and is the key enzyme of gluconeogenesis in the liver. The encoded protein may serve a similar function, although it is constitutively expressed and not modulated by hormones such as glucagon and insulin that regulate the cytosolic form. Alternatively spliced transcript variants have been described. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-PCK2 antibody used in Western Blot (WB). GTX114919

GTX114919 WB Image

PCK2 antibody detects PCK2 protein by western blot analysis.
A. 30 μg Neuro2A whole cell lysate/extract
B. 30 μg GL261 whole cell lysate/extract
7.5% SDS-PAGE
PCK2 antibody (GTX114919) dilution: 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-PCK2 antibody used in Western Blot (WB). GTX114919

GTX114919 WB Image

PCK2 antibody detects PCK2 protein by western blot analysis.
A. 30 μg PC-12 whole cell lysate/extract
7.5% SDS-PAGE
PCK2 antibody (GTX114919) dilution: 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-PCK2 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX114919

GTX114919 ICC/IF Image

PCK2 antibody detects PCK2 protein at mitochondria by immunofluorescent analysis.
Sample: HepG2 cells were fixed in MeOH for 5 min.
Green: PCK2 protein stained by PCK2 antibody (GTX114919) diluted at 1:500.
Blue: Hoechst 33342 staining.
Scale bar = 10 μm.

Anti-PCK2 antibody used in Western Blot (WB). GTX114919

GTX114919 WB Image

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with PCK2 antibody (GTX114919) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-PCK2 antibody used in IHC (Paraffin sections) (IHC-P). GTX114919

GTX114919 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human breast cancer, using PCK2(GTX114919) antibody at 1:250 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-PCK2 antibody used in Western Blot (WB). GTX114919

GTX114919 WB Image

KO/KD

The data was published in the journal Oncotarget in 2017. PMID: 29262588

REFERENCE

Application Reference

Hsu HP et al. Cancer Med 2023; 12 (2) : 1588-1601 Mitochondrial phosphoenolpyruvate carboxykinase promotes tumor growth in estrogen receptor-positive breast cancer via regulation of the mTOR pathway.
Chu PY et al. Oncotarget 2017; 8 (61) : 103613-103625 Mitochondrial phosphoenolpyruvate carboxykinase (PEPCK-M) regulates the cell metabolism of pancreatic neuroendocrine tumors (pNET) and de-sensitizes pNET to mTOR inhibitors.
Application : WB
Reactivity : Human
Submit a Reference

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169