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PCK2 antibody

Anti-PCK2 antibody used in IHC (Paraffin sections) (IHC-P). GTX114919
Anti-PCK2 antibody used in Western Blot (WB). GTX114919
Anti-PCK2 antibody used in Western Blot (WB). GTX114919
Anti-PCK2 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX114919
Anti-PCK2 antibody used in Western Blot (WB). GTX114919

Cat No. GTX114919

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF, IHC-P

Reactivity

Human, Mouse, Rat
Package
100 μl ($329),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

71 kDa. ( Note )

Positive Control

293T , HeLa , HepG2 , Neuro2A , GL261 , PC-12

Predict Reactivity

Bovine, Pig, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the N-terminus region of human PCK2. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_10621116

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

phosphoenolpyruvate carboxykinase 2, mitochondrial , PEPCK , PEPCK-M , PEPCK2

Cellular Localization

Mitochondrion

Background

This gene encodes a member of the phosphoenolpyruvate carboxykinase (GTP) family. The protein is a mitochondrial enzyme that catalyzes the conversion of oxaloacetate to phosphoenolpyruvate in the presence of GTP. A cytosolic form encoded by a different gene has also been characterized and is the key enzyme of gluconeogenesis in the liver. The encoded protein may serve a similar function, although it is constitutively expressed and not modulated by hormones such as glucagon and insulin that regulate the cytosolic form. Alternatively spliced transcript variants have been described. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-PCK2 antibody used in IHC (Paraffin sections) (IHC-P). GTX114919

GTX114919 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human breast cancer, using PCK2(GTX114919) antibody at 1:250 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-PCK2 antibody used in Western Blot (WB). GTX114919

GTX114919 WB Image

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with PCK2 antibody (GTX114919) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-PCK2 antibody used in Western Blot (WB). GTX114919

GTX114919 WB Image

PCK2 antibody detects PCK2 protein by western blot analysis.
A. 30 μg Neuro2A whole cell lysate/extract
B. 30 μg GL261 whole cell lysate/extract
7.5% SDS-PAGE
PCK2 antibody (GTX114919) dilution: 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-PCK2 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX114919

GTX114919 ICC/IF Image

PCK2 antibody detects PCK2 protein at mitochondria by immunofluorescent analysis.
Sample: HepG2 cells were fixed in MeOH for 5 min.
Green: PCK2 protein stained by PCK2 antibody (GTX114919) diluted at 1:500.
Blue: Hoechst 33342 staining.
Scale bar = 10 μm.

Anti-PCK2 antibody used in Western Blot (WB). GTX114919

GTX114919 WB Image

PCK2 antibody detects PCK2 protein by western blot analysis.
A. 30 μg PC-12 whole cell lysate/extract
7.5% SDS-PAGE
PCK2 antibody (GTX114919) dilution: 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

REVIEW

PCK2 antibody Cat No. GTX114919

Rating ( Average 5 based on 1 users reviews)
Application
Western Blot(WB) ( Average 5 based on 1 users reviews)
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : WB
Sample Species : Ms
Figure Note : The data was peer reviewed and published in the journal Oncotarget in 2017.PMID: 29262588
SDS
Glycerol.pdf
Thimerosal.pdf