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PCK2 antibody

Anti-PCK2 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX64476
Anti-PCK2 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX64476
Anti-PCK2 antibody used in Western Blot (WB). GTX64476
Anti-PCK2 antibody used in IHC (Paraffin sections) (IHC-P). GTX64476
Anti-PCK2 antibody used in IHC (Paraffin sections) (IHC-P). GTX64476
Anti-PCK2 antibody used in Immunoprecipitation (IP). GTX64476

Cat No. GTX64476

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P, IP

Reactivity

 Human, Mouse, Rat
Package
100 μl ($339)
See all PCK2 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500 - 1:2000
ICC/IF 1:50 - 1:200
IHC-P 1:50 - 1:100
IP 1:20 - 1:100
Not tested in other applications.

Calculated MW

71 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS pH7.3, 0.02% sodium azide, 50% glycerol.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1-280 of human PCK2 (NP_004554.2).

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

phosphoenolpyruvate carboxykinase 2, mitochondrial , PEPCK , PEPCK-M , PEPCK2

Cellular Localization

Mitochondrion

Background

This gene encodes a mitochondrial enzyme that catalyzes the conversion of oxaloacetate to phosphoenolpyruvate in the presence of guanosine triphosphate (GTP). A cytosolic form of this protein is encoded by a different gene and is the key enzyme of gluconeogenesis in the liver. Alternatively spliced transcript variants have been described. [provided by RefSeq, Apr 2014]

Database

Research Area

DATA IMAGES

Anti-PCK2 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX64476

GTX64476 ICC/IF Image

ICC/IF analysis of NIH/3T3 cells using GTX64476 PCK2 antibody.
Blue : DAPI
Dilution : 1:100

Anti-PCK2 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX64476

GTX64476 ICC/IF Image

ICC/IF analysis of U2OS cells using GTX64476 PCK2 antibody.
Blue : DAPI
Dilution : 1:100

Anti-PCK2 antibody used in Western Blot (WB). GTX64476

GTX64476 WB Image

WB analysis of various sample lysates using GTX64476 PCK2 antibody. The signal was developed with ECL plus-Enhanced.
Dilution : 1:1000
Loading : 25μg per lane

Anti-PCK2 antibody used in IHC (Paraffin sections) (IHC-P). GTX64476

GTX64476 IHC-P Image

IHC-P analysis of mouse kidney tissue using GTX64476 PCK2 antibody.
Dilution : 1:100

Anti-PCK2 antibody used in IHC (Paraffin sections) (IHC-P). GTX64476

GTX64476 IHC-P Image

IHC-P analysis of rat kidney tissue using GTX64476 PCK2 antibody.
Dilution : 1:100

Anti-PCK2 antibody used in Immunoprecipitation (IP). GTX64476

GTX64476 IP Image

IP analysis of HepG2 cell lysate using GTX64476 PCK2 antibody.
Dilution : 1:1000

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 339