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PCM1 antibody, Internal

Anti-PCM1 antibody, Internal used in IHC (Paraffin sections) (IHC-P). GTX81421
Anti-PCM1 antibody, Internal used in Western Blot (WB). GTX81421
Anti-PCM1 antibody, Internal used in Flow cytometry (FACS). GTX81421
Anti-PCM1 antibody, Internal used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX81421

Cat. No. GTX81421

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P, FACS

Reactivity

 Human
Package
400 μl ($399)
See all PCM1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:1000
ICC/IF 1:10-1:50
IHC-P 1:50-1:100
FACS 1:10-1:50
Not tested in other applications.

Calculated MW

229 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

0.09% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

KLH conjugated synthetic peptide between 632-661 amino acids from the Central region of human PCM-1.

Purification

Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

pericentriolar material 1 , PTC4 , RET/PCM-1

Cellular Localization

Cytoplasm, cytoskeleton

Background

The protein encoded by this gene is a component of centriolar satellites, which are electron dense granules scattered around centrosomes. Inhibition studies show that this protein is essential for the correct localization of several centrosomal proteins, and for anchoring microtubules to the centrosome. Chromosomal aberrations involving this gene are associated with papillary thyroid carcinomas and a variety of hematological malignancies, including atypical chronic myeloid leukemia and T-cell lymphoma. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2015]

Database

Research Area

DATA IMAGES

Anti-PCM1 antibody, Internal used in IHC (Paraffin sections) (IHC-P). GTX81421

GTX81421 IHC-P Image

IHC-P analysis of human breast carcinoma using GTX81421 PCM1 antibody, Internal.

Anti-PCM1 antibody, Internal used in Western Blot (WB). GTX81421

GTX81421 WB Image

WB analysis of Ramos cell lysate (35ug/lane) using GTX81421 PCM1 antibody, Internal.

Anti-PCM1 antibody, Internal used in Flow cytometry (FACS). GTX81421

GTX81421 FACS Image

FACS analysis of Ramos cells using GTX81421 PCM1 antibody, Internal.
Top histogram : negative control
Bottom histogram : Ramos cells

Anti-PCM1 antibody, Internal used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX81421

GTX81421 ICC/IF Image

ICC/IF analysis of A549 cells using GTX81421 PCM1 antibody, Internal.
Green : PCM-1
Red : Actin filament
Blue : cell nucleus
Fixation : 4% paraformaldehyde (PFA) (20 min)
Permeabilization : Triton X-100 (0.1%, 10 min)
Dilution : 1:25

REFERENCE

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REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399