GeneTex
United States (US)

PCMT1 antibody

GTX33392_20180530_IP_18121410_852.jpg
GTX33392_20180530_WB_18121410_359.jpg
GTX33392_20180530_ICCIF_18121410_617.jpg

Cat No. GTX33392

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IP
Reactivity Human, Mouse, Rat
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500 - 1:2000
ICC/IF 1:50 - 1:200
IP Assay dependent
Not tested in other applications.
PROPERTIES

Form

Liquid

Buffer

PBS pH7.3, 0.02% sodium azide, 50% glycerol.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Antigen Species

Human

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 62-286 of human PCMT1 (NP_001238978.1).

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Protein-L-Isoaspartate (D-Aspartate) O-Methyltransferase,Pimt,Pcmt1

Background

This gene encodes a member of the type II class of protein carboxyl methyltransferase enzymes. The encoded enzyme plays a role in protein repair by recognizing and converting D-aspartyl and L-isoaspartyl residues resulting from spontaneous deamidation back to the normal L-aspartyl form. The encoded protein may play a protective role in the pathogenesis of Alzheimer's disease, and single nucleotide polymorphisms in this gene have been associated with spina bifida and premature ovarian failure. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Oct 2011]

Research Area

DATA IMAGES
GTX33392_20180530_IP_18121410_852.jpg

GTX33392 IP Image

IP analysis of HepG2 cell lysate using GTX33392 PCMT1 antibody.
IP reaction : 1μg antibody / 200μg cell lysate
Dilution : 1:1000

GTX33392_20180530_WB_18121410_359.jpg

GTX33392 WB Image

WB analysis of various samples using GTX33392 PCMT1 antibody.
Loading : 25μg per lane

GTX33392_20180530_ICCIF_18121410_617.jpg

GTX33392 ICC/IF Image

ICC/IF analysis of HeLa cell using GTX33392 PCMT1 antibody.
Blue : DAPI

Package List Price ($)
$ 329