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PCMT1 antibody

Anti-PCMT1 antibody used in Immunoprecipitation (IP). GTX33392
Anti-PCMT1 antibody used in Western Blot (WB). GTX33392
Anti-PCMT1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX33392

Cat No. GTX33392

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF, IP

Reactivity

Human, Mouse, Rat
Package
100 μl ($329)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500 - 1:2000
ICC/IF 1:50 - 1:200
IP Assay dependent
Not tested in other applications.

Calculated MW

25 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS pH7.3, 0.02% sodium azide, 50% glycerol.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Antigen Species

Human

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 62-286 of human PCMT1 (NP_001238978.1).

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

protein-L-isoaspartate (D-aspartate) O-methyltransferase , PIMT

Cellular Localization

Cytoplasm

Background

This gene encodes a member of the type II class of protein carboxyl methyltransferase enzymes. The encoded enzyme plays a role in protein repair by recognizing and converting D-aspartyl and L-isoaspartyl residues resulting from spontaneous deamidation back to the normal L-aspartyl form. The encoded protein may play a protective role in the pathogenesis of Alzheimer's disease, and single nucleotide polymorphisms in this gene have been associated with spina bifida and premature ovarian failure. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Oct 2011]

Database

Research Area

DATA IMAGES

Anti-PCMT1 antibody used in Immunoprecipitation (IP). GTX33392

GTX33392 IP Image

IP analysis of HepG2 cell lysate using GTX33392 PCMT1 antibody.
IP reaction : 1μg antibody / 200μg cell lysate
Dilution : 1:1000

Anti-PCMT1 antibody used in Western Blot (WB). GTX33392

GTX33392 WB Image

WB analysis of various samples using GTX33392 PCMT1 antibody.
Loading : 25μg per lane

Anti-PCMT1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX33392

GTX33392 ICC/IF Image

ICC/IF analysis of HeLa cell using GTX33392 PCMT1 antibody.
Blue : DAPI

REFERENCE

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REVIEW

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Package List Price ($)
$ 329