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PCMT1 antibody

Anti-PCMT1 antibody used in Western Blot (WB). GTX33392
Anti-PCMT1 antibody used in Immunoprecipitation (IP). GTX33392
Anti-PCMT1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX33392

Cat. No. GTX33392

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IP

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399)
See all PCMT1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500 - 1:2000
ICC/IF 1:50 - 1:100
IP 1:50 - 1:200
Not tested in other applications.

Calculated MW

25 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 62-286 of human PCMT1 (NP_001238978.1).

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

protein-L-isoaspartate (D-aspartate) O-methyltransferase , PIMT

Cellular Localization

Cytoplasm

Background

This gene encodes a member of the type II class of protein carboxyl methyltransferase enzymes. The encoded enzyme plays a role in protein repair by recognizing and converting D-aspartyl and L-isoaspartyl residues resulting from spontaneous deamidation back to the normal L-aspartyl form. The encoded protein may play a protective role in the pathogenesis of Alzheimer's disease, and single nucleotide polymorphisms in this gene have been associated with spina bifida and premature ovarian failure. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Oct 2011]

Database

Research Area

DATA IMAGES

Anti-PCMT1 antibody used in Western Blot (WB). GTX33392

GTX33392 WB Image

WB analysis of various sample lysates using GTX33392 PCMT1 antibody.
Loading : 25μg per lane

Anti-PCMT1 antibody used in Immunoprecipitation (IP). GTX33392

GTX33392 IP Image

IP analysis of HepG2 cell lysate using GTX33392 PCMT1 antibody.
Antibody amount : 1μg / 200μg lysate
Dilution : 1:1000

Anti-PCMT1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX33392

GTX33392 ICC/IF Image

ICC/IF analysis of HeLa cells using GTX33392 PCMT1 antibody.
Blue : DAPI

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399