PCNA antibody [6B12-D7-H9]

Cat. No. GTX49256

Host	Mouse
Clonality	Monoclonal
Clone Name	6B12-D7-H9
Isotype	IgG2b
Application	WB, ICC/IF, IP
Reactivity	Human, Mouse, Rat, Hamster, Monkey
Package	100 μg ($399)

See all PCNA products

Datasheet

View product citations for antibody GTX49256 on CiteAb

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Recommended Dilution
WB	Assay dependent
ICC/IF	Assay dependent
IP	Assay dependent

Not tested in other applications.

Calculated MW

29 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 0.5% BSA, 50% glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Purified recombinant human PCNA protein fragments expressed in E.coli.

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

proliferating cell nuclear antigen , ATLD2

Cellular Localization

Nucleus

Background

The protein encoded by this gene is found in the nucleus and is a cofactor of DNA polymerase delta. The encoded protein acts as a homotrimer and helps increase the processivity of leading strand synthesis during DNA replication. In response to DNA damage, this protein is ubiquitinated and is involved in the RAD6-dependent DNA repair pathway. Two transcript variants encoding the same protein have been found for this gene. Pseudogenes of this gene have been described on chromosome 4 and on the X chromosome. [provided by RefSeq, Jul 2008]

Database

Gene ID: 5111 PCNA
UniProt: P12004 PCNA

Research Area

DATA IMAGES

GTX49256 IP Image

IP analysis of HeLa lysates using control IgG and PCNA antibody. The precipitates were detected by the same PCNA antibody.

GTX49256 ICC/IF Image

ICC/IF analysis of HeLa cells using PCNA antibody at a dilution of 1:100.

GTX49256 WB Image

WB analysis of indicated lysates using PCNA antibody at a dilution of 1:1,000.

REFERENCE

There are currently no references for PCNA antibody [6B12-D7-H9] (GTX49256). Be the first to share your publications with this product.

Submit a Reference

REVIEW

There are currently no reviews for PCNA antibody [6B12-D7-H9] (GTX49256). Be the first to share your experience with this product.

Submit a Review

Package

List Price ($)

100 μg

$ 399

Qty