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PCNA antibody [7H4F8]

Anti-PCNA antibody [7H4F8] used in ELISA (ELISA). GTX60692
Anti-PCNA antibody [7H4F8] used in Western Blot (WB). GTX60692
Anti-PCNA antibody [7H4F8] used in IHC (Paraffin sections) (IHC-P). GTX60692
Anti-PCNA antibody [7H4F8] used in Western Blot (WB). GTX60692
Anti-PCNA antibody [7H4F8] used in Flow cytometry (FACS). GTX60692
Anti-PCNA antibody [7H4F8] used in IHC (Paraffin sections) (IHC-P). GTX60692

Cat. No. GTX60692

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 7H4F8

Isotype

 IgG1

Application

 WB, IHC-P, FACS, ELISA

Reactivity

 Human
Package
100 μg ($409)
See all PCNA products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1/500 - 1/2000
IHC-P 1/200 - 1/1000
FACS 1/200 - 1/400
ELISA 1/10000
Not tested in other applications.

Calculated MW

29 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

0.05% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Purified recombinant fragment of human PCNA (AA: 53-196 ) expressed in E. Coli.

Purification

Protein G Purified

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

proliferating cell nuclear antigen , ATLD2

Cellular Localization

Nucleus

Background

The protein encoded by this gene is found in the nucleus and is a cofactor of DNA polymerase delta. The encoded protein acts as a homotrimer and helps increase the processivity of leading strand synthesis during DNA replication. In response to DNA damage, this protein is ubiquitinated and is involved in the RAD6-dependent DNA repair pathway. Two transcript variants encoding the same protein have been found for this gene. Pseudogenes of this gene have been described on chromosome 4 and on the X chromosome. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-PCNA antibody [7H4F8] used in ELISA (ELISA). GTX60692

GTX60692 ELISA Image

ELISA analysis of antigen using GTX60692 PCNA antibody [7H4F8].
Black : Control antigen 100ng
Purple : Antigen 10ng
Blue : Antigen 50ng
Red : Antigen 100ng

Anti-PCNA antibody [7H4F8] used in Western Blot (WB). GTX60692

GTX60692 WB Image

WB analysis of HEK293 (1) and PCNA (AA: 53-196)-hIgGFc transfected HEK293 (2) cell lysate using GTX60692 PCNA antibody [7H4F8].

Anti-PCNA antibody [7H4F8] used in IHC (Paraffin sections) (IHC-P). GTX60692

GTX60692 IHC-P Image

IHC-P analysis of cervical cancer tissue using GTX60692 PCNA antibody [7H4F8].

Anti-PCNA antibody [7H4F8] used in Western Blot (WB). GTX60692

GTX60692 WB Image

WB analysis of A431 (1), HeLa (2), HepG2 (3), Raji (4), and MOLT4 (5) cell lysate using GTX60692 PCNA antibody [7H4F8].

Anti-PCNA antibody [7H4F8] used in Flow cytometry (FACS). GTX60692

GTX60692 FACS Image

FACS analysis of MOLT4 cells using GTX60692 PCNA antibody [7H4F8].
Green : PCNA
Purple : negative control

Anti-PCNA antibody [7H4F8] used in IHC (Paraffin sections) (IHC-P). GTX60692

GTX60692 IHC-P Image

IHC-P analysis of colon cancer tissue using GTX60692 PCNA antibody [7H4F8].

REFERENCE

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REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 409