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PCNA antibody, C-term

Anti-PCNA antibody, C-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX77666
Anti-PCNA antibody, C-term used in Western Blot (WB). GTX77666
Anti-PCNA antibody, C-term used in IHC (Paraffin sections) (IHC-P). GTX77666
Anti-PCNA antibody, C-term used in Western Blot (WB). GTX77666

Cat. No. GTX77666

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human
Package
100 μg ($399)
See all PCNA products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 0.2-2.5 ug/ml
ICC/IF Assay dependent
IHC-P 2-10 ug/ml
Not tested in other applications.

Calculated MW

29 kDa. ( Note )

Positive Control

HepG2 , NT-2

PROPERTIES

Form

Liquid

Buffer

PBS, 2% Sucrose

Preservative

0.09% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.5-1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

A synthetic peptide corresponding to a C-terminal region of Human PCNA

Purification

Protein A purified

Conjugation

Unconjugated

RRID

AB_424614

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

proliferating cell nuclear antigen , ATLD2

Cellular Localization

Nucleus

Background

The protein encoded by this gene is found in the nucleus and is a cofactor of DNA polymerase delta. The encoded protein acts as a homotrimer and helps increase the processivity of leading strand synthesis during DNA replication. In response to DNA damage, this protein is ubiquitinated and is involved in the RAD6-dependent DNA repair pathway. Two transcript variants encoding the same protein have been found for this gene. Pseudogenes of this gene have been described on chromosome 4 and on the X chromosome. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-PCNA antibody, C-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX77666

GTX77666 ICC/IF Image

ICC/IF analysis of NT-2 cells using GTX77666 PCNA antibody at 1:500.
Red : PCNA
Blue : DAPI

Anti-PCNA antibody, C-term used in Western Blot (WB). GTX77666

GTX77666 WB Image

WB analysis of NT-2 cells using GTX77666 PCNA antibody at 2μg/ml.

Anti-PCNA antibody, C-term used in IHC (Paraffin sections) (IHC-P). GTX77666

GTX77666 IHC-P Image

IHC-P analysis of human spleen tissue using GTX77666 PCNA antibody at 4.0-8.0μg/ml.

Anti-PCNA antibody, C-term used in Western Blot (WB). GTX77666

GTX77666 WB Image

WB analysis of HepG2 cells using GTX77666 PCNA antibody at 1.25μg/ml.

REFERENCE

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REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399