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PCNA antibody [PC10] (FITC)

Anti-PCNA antibody [PC10] (FITC) used in Flow cytometry (FACS). GTX75144

Cat No. GTX75144

Host

Mouse

Clonality

Monoclonal

Clone Name

PC10

Isotype

IgG2a

Application

FACS, IHC

Reactivity

Human, Mouse, Rat, Rabbit, Sheep, Cat, Dog, Hamster, Chicken, Atlantic salmon, Cynomolgus monkey, Ferret, Horse, Rhesus Monkey, Xenopus
Package
50 μg ($319)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
FACS Neat-1/10
IHC Assay dependent

Note :

FACS
Membrane permeabilization is required for this application. Use 10μl of the suggested working dilution to label 106 cells in 100μl.

Not tested in other applications.

Calculated MW

29 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 0.09% Sodium Azide, 1% BSA

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. Protect from light.

Concentration

0.1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Rat

Immunogen

Rat PCNA made in the protein A expression vector pR1T2T

Purification

Protein G purified
From tissue culture supernatant

Conjugation

Fluorescein isothiocyanate (FITC)

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

Proliferating Cell Nuclear Antigen , Pcnar , Pcna/Cyclin , Pcna

Background

cell cycle protein that may contribute to the repair of DNA damage and to cell proliferation caused by exposure to pulmonary toxicants; may influence cell survival in ischaemically compromised tissue [RGD, Feb 2006]

Database

Research Area

DATA IMAGES

Anti-PCNA antibody [PC10] (FITC) used in Flow cytometry (FACS). GTX75144

GTX75144 FACS Image

FACS analysis of KM-H2 cells using GTX75144 PCNA antibody [PC10] (FITC).

REFERENCE

REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf