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PCNA antibody [PC10] (FITC)

Anti-PCNA antibody [PC10] (FITC) used in Flow cytometry (FACS). GTX75144

Cat. No. GTX75144

Reference 24

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 PC10

Isotype

 IgG2a

Application

 FACS, IHC

Reactivity

 Human, Mouse, Rat, Rabbit, Sheep, Cat, Dog, Hamster, Chicken, Atlantic salmon, Cynomolgus monkey, Ferret, Horse, Rhesus Monkey, Xenopus
Package
50 μg ($399)
See all PCNA products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
FACS Neat-1/10
IHC Assay dependent

Note :

FACS
Membrane permeabilization is required for this application. Use 10μl of the suggested working dilution to label 106 cells in 100μl.
Not tested in other applications.

Calculated MW

29 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA

Preservative

0.09% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. Protect from light.

Concentration

0.1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Rat

Immunogen

Rat PCNA made in the protein A expression vector pR1T2T

Purification

Protein G purified
From tissue culture supernatant

Conjugation

Fluorescein isothiocyanate (FITC)

RRID

AB_376619

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

proliferating cell nuclear antigen , PCNAR , Pcna/cyclin

Cellular Localization

Nucleus

Background

cell cycle protein that may contribute to the repair of DNA damage and to cell proliferation caused by exposure to pulmonary toxicants; may influence cell survival in ischaemically compromised tissue [RGD, Feb 2006]

Database

Research Area

DATA IMAGES

Anti-PCNA antibody [PC10] (FITC) used in Flow cytometry (FACS). GTX75144

GTX75144 FACS Image

FACS analysis of KM-H2 cells using GTX75144 PCNA antibody [PC10] (FITC).

REFERENCE

Clone Reference

Yeom M et al. Chem Res Toxicol 2020; 33 (8):2120-2129 Three Human Pol ι Variants with Impaired Polymerase Activity Fail to Rescue H2O2 Sensitivity in POLI-Deficient Cells.
Bryche B et al. Neural Regen Res 2020; 15 (2):352-360 Endothelin increases the proliferation of rat olfactory mucosa cells.
Ketteler J et al. J Clin Med 2019; 8 (3) Progression-Related Loss of Stromal Caveolin 1 Levels Mediates Radiation Resistance in Prostate Carcinoma via the Apoptosis Inhibitor TRIAP1.
Bryche B et al. Brain Behav Immun 2019; (Epub) IL-17c is involved in olfactory mucosa responses to Poly(I:C) mimicking virus presence.
?ensoy E et al. Turk J Gastroenterol 2019; 30 (10):917-924 Determination of the changes on the small intestine of pregnant mice by histological, enzyme histochemical, and immunohistochemical methods.
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Application Reference
Tomas J et al. FASEB J 2013; 27 (2):645-55 Primocolonization is associated with colonic epithelial maturation during conventionalization.
Application : IHC
Reactivity : Rat
Clone Reference
Yeom M et al. Chem Res Toxicol 2020; 33 (8):2120-2129 Three Human Pol ι Variants with Impaired Polymerase Activity Fail to Rescue H2O2 Sensitivity in POLI-Deficient Cells.
Bryche B et al. Neural Regen Res 2020; 15 (2):352-360 Endothelin increases the proliferation of rat olfactory mucosa cells.
Ketteler J et al. J Clin Med 2019; 8 (3) Progression-Related Loss of Stromal Caveolin 1 Levels Mediates Radiation Resistance in Prostate Carcinoma via the Apoptosis Inhibitor TRIAP1.
Bryche B et al. Brain Behav Immun 2019; (Epub) IL-17c is involved in olfactory mucosa responses to Poly(I:C) mimicking virus presence.
?ensoy E et al. Turk J Gastroenterol 2019; 30 (10):917-924 Determination of the changes on the small intestine of pregnant mice by histological, enzyme histochemical, and immunohistochemical methods.
Chauvigne F et al. PLoS One 2017; 12 (3):e0174387 toward developing recombinant *-based hormone therapies for increasing fertility in the flatfish senegalese sole.
Chauvigne F et al. Comp Biochem Physiol A Mol Integr Physiol 2017; 206:36-46 Dual estrogenic regulation of the nuclear progestin receptor and spermatogonial renewal during gilthead seabream (Sparus aurata) spermatogenesis.
Riviere S et al. Sci Rep 2016; 6:34011 High Fructose Diet inducing diabetes rapidly impacts olfactory epithelium and behavior in mice.
Francois A et al. Sci Rep 2016; 6:24687 Olfactory epithelium changes in germfree mice.
Ozaydin T et al. Microsc Res Tech 2016; Immunohistochemical distribution of heat shock protein 70 and proliferating cell nuclear antigen in mouse placenta at different gestational stages.
Gu LH et al. Cell Mol Immunol 2015; 12 (4):444-55 Immunogenicity of allogeneic mesenchymal stem cells transplanted via different routes in diabetic rats.
Boj M et al. PLoS One 2015; 10 (11):e0142512 *-activated androgen-dependent and independent pathways regulate aquaporin expression during teleost (sparus aurata) spermatogenesis.
Aurelien Raynaud et al. Behavioural Brain Research 2015; 15 (291):36-45 Chronic variable stress exposure in male Wistar rats affects the first step of olfactory detection
Liu Y et al. Cytotechnology 2014; Lupeol induces apoptosis and inhibits invasion in gallbladder carcinoma GBC-SD cells by suppression of EGFR/MMP-9 signaling pathway.
Turpin W et al. PLoS One 2013; 8 (4):e57711 Behavior of lactobacilli isolated from fermented slurry (ben-saalga) in gnotobiotic rats.
Gultekin FA et al. J Invest Surg 2013; 26 (5):242-52 Effects of ozone oxidative preconditioning on liver regeneration after partial hepatectomy in rats.
Rul F et al. J Biol Chem 2011; 286 (12):10288-96 Impact of the metabolic activity of Streptococcus thermophilus on the colon epithelium of gnotobiotic rats.
Cherbuy C et al. Am J Physiol Gastrointest Liver Physiol 2010; 299 (2):G348-57 Microbiota matures colonic epithelium through a coordinated induction of cell cycle-related proteins in gnotobiotic rat.
Guo Z et al. Nat Chem Biol 2010; 6 (10):766-73 Methylation of FEN1 suppresses nearby phosphorylation and facilitates PCNA binding.
Ji RC et al. Microvasc Res 2010; 80 (2):195-201 Multiple expressions of lymphatic markers and morphological evolution of newly formed lymphatics in lymphangioma and lymph node lymphangiogenesis.
Kumar R et al. Clin Cancer Res 2009; 15 (12):4123-30 extranuclear coactivator signaling confers insensitivity to *.
Yasemin O et al. Journal of Animal and Veterinary Advances. 2009;:8(9):1694-701. Comparative Skin Histology of the White New Zealand and Angora Rabbits: Histometrical and Immunohistochemical Evaluations.
Application : IHC
Krasna L et al. Breast Cancer Res Treat 2002; 71 (3):219-35 Large expansion of morphologically heterogeneous mammary epithelial cells, including the luminal phenotype, from human breast tumours.
SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399