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PCNA antibody [PCNA/694]

Anti-PCNA antibody [PCNA/694] used in Western Blot (WB). GTX34964
Anti-PCNA antibody [PCNA/694] used in IHC (Paraffin sections) (IHC-P). GTX34964

Cat No. GTX34964

Host Mouse
Clonality Monoclonal
Clone Name PCNA/694
Isotype IgG2a
Application WB, ICC/IF, IHC-P
Reactivity Human
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 0.5-1ug/ml
ICC/IF 0.5-1ug/ml
IHC-P 0.25-0.5ug/ml for 30 minutes at RT

Note :

IHC-P
Boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes

Not tested in other applications.

Calculated MW

29 kDa. ( Note )

Specificity/Sensitivity

Recognizes a non-histone protein of 36kDa, which is identified as proliferating cell nuclear antigen (PCNA). It is also known as cyclin or polymerase delta auxiliary protein. Elevated expression of PCNA/cyclin has been shown in the nucleus during late G1 phase immediately before the onset of DNA synthesis, becoming maximal during S-phase and declining during G2 and M phases. This MAb is excellent for multiple applications.
PROPERTIES

Form

Liquid

Buffer

Prepared in 10mM PBS with 0.05% BSA and 0.05% azide.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Antigen Species

Human

Immunogen

Recombinant full length human PCNA protein

Purification

Ab purified from Bioreactor Concentrate by Protein A/G

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Proliferating Cell Nuclear Antigen,Atld2,Pcna

Background

The protein encoded by this gene is found in the nucleus and is a cofactor of DNA polymerase delta. The encoded protein acts as a homotrimer and helps increase the processivity of leading strand synthesis during DNA replication. In response to DNA damage, this protein is ubiquitinated and is involved in the RAD6-dependent DNA repair pathway. Two transcript variants encoding the same protein have been found for this gene. Pseudogenes of this gene have been described on chromosome 4 and on the X chromosome. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES
Anti-PCNA antibody [PCNA/694] used in Western Blot (WB). GTX34964

GTX34964 WB Image

Western Blot Analysis of HepG2 Cell Lysate using PCNA Monoclonal Antibody (PCNA/694)

Anti-PCNA antibody [PCNA/694] used in IHC (Paraffin sections) (IHC-P). GTX34964

GTX34964 IHC-P Image

Formalin-fixed, paraffin-embedded human Tonsil stained with PCNA Monoclonal Antibody (PCNA/694)

Package List Price ($)
$ 329