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PCNA antibody

Anti-PCNA antibody used in Western Blot (WB). GTX00723
Anti-PCNA antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX00723
Anti-PCNA antibody used in Western Blot (WB). GTX00723

Cat No. GTX00723

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF, IP, Dot, ELISA

Reactivity

Human, Mouse, Rat, Hamster, Chicken, Xenopus, Chinese Hamster
Package
100 μg ($319)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:1000-1:2000
ICC/IF 1:100-1:1000
IP 1:100-1:500
Dot 1:500-1:2000
ELISA 1:500-1:2000

Note :

Dot
It could detect 0.3 ng of PCNA.

Not tested in other applications.

Calculated MW

29 kDa. ( Note )

Specificity/Sensitivity

This antibody could detect 0.3 ng of PCNA by dot blot.

PROPERTIES

Form

Liquid

Buffer

Filter-sterilized PBS, 50% glycerol

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Purified recombinant human PCNA (full-size, no-tag attached)

Purification

Purified IgG

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

proliferating cell nuclear antigen , ATLD2

Cellular Localization

Nucleus

Background

The protein encoded by this gene is found in the nucleus and is a cofactor of DNA polymerase delta. The encoded protein acts as a homotrimer and helps increase the processivity of leading strand synthesis during DNA replication. In response to DNA damage, this protein is ubiquitinated and is involved in the RAD6-dependent DNA repair pathway. Two transcript variants encoding the same protein have been found for this gene. Pseudogenes of this gene have been described on chromosome 4 and on the X chromosome. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-PCNA antibody used in Western Blot (WB). GTX00723

GTX00723 WB Image

WB analysis of various samples using GTX00723 PCNA antibody.
Lane 1 : Full-length recombinant human PCNA protein (5 ng)
Lane 2 : HeLa whole cell lysate (20 μg)
Lane 3 : MCF-7 whole cell lysate (20 μg)
Lane 4 : NIH3T3 whole cell lysate (20 μg)
Lane 5 : CHO whole cell lysate (20 μg)
Lane 6 : Xenopus laevis egg (frog; 20 μg)
Dilution : 1:1000

Anti-PCNA antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX00723

GTX00723 ICC/IF Image

ICC/IF analysis of GM0637 cells using GTX00723 PCNA antibody.
Green : Primary antibody
Blue : Hoechst 33342
Dilution : 1:100
Fixation : 4% PFA
Permeabilization : 0.5% Triton X-100

Anti-PCNA antibody used in Western Blot (WB). GTX00723

GTX00723 WB Image

WB analysis of various samples using GTX00723 PCNA antibody.
Lane 1 : HeLa whole cell lysate
Lane 2 : Xenopus egg extract
Dilution : 1:1000
Loading : 20 μg

REFERENCE

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SDS
PBS.pdf
Glycerol.pdf