Summary
PCNA antibody recognizes the proliferating cell nuclear antigen (PCNA), a cofactor of DNA polymerase delta with a predicted molecular weight of 29 kDa. PCNA is important for DNA synthesis, repair, and epigenetic modifications, and also serves as an organelle marker of the nucleus. PCNA antibody can be used for distinguishing between early, middle, and late S-phase of the cell cycle as well as grading of different neoplasms.
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
Application |
Recommended Dilution |
1:500-1:3000 |
1:100-1:1000 |
1:100-1:1000 |
Assay dependent |
1:100-1:500 |
Assay dependent |
Assay dependent |
Not tested in other applications.
Calculated MW
Positive Control
NIH-3T3 , PC-12
Product Note
IP/MS validation was supported by references (PMID:30377373)
Predict Reactivity
Zebrafish, Bovine, Chicken, Pig, Xenopus tropicalis, Rhesus Monkey, Killifish(>80% identity)
Form
Liquid
Buffer
1XPBS (pH7), 1% BSA, 20% Glycerol
Preservative
0.025% ProClin 300
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
0.11 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Recombinant protein encompassing a sequence within the center region of human PCNA. The exact sequence is proprietary.
Purification
Purified by antigen-affinity chromatography.
Conjugation
Unconjugated
RRID
AB_1241163
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
Synonyms
proliferating cell nuclear antigen , ATLD2
Cellular Localization
Nucleus
Background
The protein encoded by this gene is found in the nucleus and is a cofactor of DNA polymerase delta. The encoded protein acts as a homotrimer and helps increase the processivity of leading strand synthesis during DNA replication. In response to DNA damage, this protein is ubiquitinated and is involved in the RAD6-dependent DNA repair pathway. Two transcript variants encoding the same protein have been found for this gene. Pseudogenes of this gene have been described on chromosome 4 and on the X chromosome. [provided by RefSeq]
Database
Research Area