PRODUCT
Summary
PCNA antibody recognizes the proliferating cell nuclear antigen (PCNA), a cofactor of DNA polymerase delta with a predicted molecular weight of 29 kDa. PCNA is important for DNA synthesis, repair, and epigenetic modifications, and also serves as an organelle marker of the nucleus. PCNA antibody can be used for distinguishing between early, middle, and late S-phase of the cell cycle as well as grading of different neoplasms.
APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1:500-1:3000 |
| 1:100-1:1000 |
| 1:100-1:1000 |
| Assay dependent |
| 1:100-1:500 |
| Assay dependent |
| Assay dependent |
Not tested in other applications.
Calculated MW
Positive Control
NIH-3T3 , PC-12
Product Note
IP/MS validation was supported by references (PMID:30377373)
Predict Reactivity
Zebrafish, Bovine, Chicken, Pig, Xenopus tropicalis, Rhesus Monkey, Killifish(>80% identity)
PROPERTIES
Form
Liquid
Buffer
1XPBS (pH7), 1% BSA, 20% Glycerol
Preservative
0.025% ProClin 300
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
0.11 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Recombinant protein encompassing a sequence within the center region of human PCNA. The exact sequence is proprietary.
Purification
Purified by antigen-affinity chromatography.
Conjugation
Unconjugated
RRID
AB_1241163
Note
For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET
Synonyms
proliferating cell nuclear antigen , ATLD2
Cellular Localization
Nucleus
Background
The protein encoded by this gene is found in the nucleus and is a cofactor of DNA polymerase delta. The encoded protein acts as a homotrimer and helps increase the processivity of leading strand synthesis during DNA replication. In response to DNA damage, this protein is ubiquitinated and is involved in the RAD6-dependent DNA repair pathway. Two transcript variants encoding the same protein have been found for this gene. Pseudogenes of this gene have been described on chromosome 4 and on the X chromosome. [provided by RefSeq]
Database
Research Area
DATA IMAGES
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GTX100539 WB Image
PCNA antibody detects PCNA protein by western blot analysis.
A. 30 μg NIH-3T3 whole cell lysate/extract
12% SDS-PAGE
PCNA antibody (GTX100539) dilution: 1:2500
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX100539 IHC-P Image
PCNA antibody detects PCNA protein at nucleus in human cervical carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded human cervical carcinoma.
PCNA antibody (GTX100539) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GTX100539 ICC/IF Image
PCNA antibody detects PCNA protein at nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: PCNA protein stained by PCNA antibody (GTX100539) diluted at 1:500.
Red: Phalloidin, a cytoskeleton marker, diluted at 1:200.
Scale bar = 10 μm.
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GTX100539 IHC-P Image
PCNA antibody detects PCNA protein at nucleus in human colon by immunohistochemical analysis.
Sample: Paraffin-embedded human colon .
PCNA antibody (GTX100539) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GTX100539 ICC/IF Image
PCNA antibody detects PCNA protein at nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in ice-cold MeOH for 5 min.
Green: PCNA protein stained by PCNA antibody (GTX100539) diluted at 1:1000.
Blue: Hoechst 33342 staining.
Scale bar = 10 μm.
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GTX100539 IP Image
Immunoprecipitation of PCBP2 protein from HeLa whole cell extracts using 5 μg of PCBP2 antibody (GTX100539).
Western blot analysis was performed using PCBP2 antibody (GTX100539).
EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.
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GTX100539 IHC-P Image
PCNA antibody detects PCNA protein at nucleus on mouse testis by immunohistochemical analysis.
Sample: Paraffin-embedded mouse testis.
PCNA antibody (GTX100539) dilution: 1:500.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
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GTX100539 WB Image
PCNA antibody detects PCNA protein by western blot analysis.
A. 30 μg PC-12 whole cell lysate/extract
12% SDS-PAGE
PCNA antibody (GTX100539) dilution: 1:2500
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX100539 IHC-P Image
PCNA antibody detects PCNA protein at nucleus on mouse testis by immunohistochemical analysis.
Sample: Paraffin-embedded mouse testis.
PCNA antibody (GTX100539) dilution: 1:500.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
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GTX100539 IHC-P Image
PCNA antibody detects PCNA protein at nucleus on rat hind brain by immunohistochemical analysis.
Sample: Paraffin-embedded rat hind brain.
PCNA antibody (GTX100539) dilution: 1:500.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
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GTX100539 WB Image
PCNA antibody detects PCNA protein by Western blot analysis. Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with PCNA antibody (GTX100539) diluted at 1:2500.
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REFERENCE
REVIEW
PCNA antibody
Cat. No. GTX100539
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Rating
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( Average 4.6 based on 14 users reviews)
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Application
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Western Blot(WB)
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( Average 4.7 based on 9 users reviews)
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Immunocytochemistry/ Immunofluorescence(ICC/IF)
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( Average 4 based on 3 users reviews)
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IHC (Paraffin sections)(IHC-P)
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( Average 5 based on 2 users reviews)
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Date :
Anonymous submitted on 14-Feb-2023
Application Tested :
ICC/IF
Sample Species :
Hu
Sample :
HEK293 overexpressing PCNA cells
Blocking :
5% non-fat milk in PBST, 25°C, 1Hr
Fixation :
4% PFA
Permeabilization /Antigen retrieval :
0.3% Triton X-100
Primary Antibody :
1:375, 16°C, 4Hr
Date :
Anonymous submitted on 08-Aug-2022
Application Tested :
WB
Sample Species :
Hu
Sample :
A375 cells
Amount used :
30μg
Blocking :
5% non-fat milk, BSA in TBST, 4°C, 1Hr
Primary Antibody :
1:2500, 4°C, > 12Hr
Date :
Anonymous submitted on 07-Feb-2022
Application Tested :
ICC/IF
Sample Species :
Ms
Sample :
WAZ-2T +SA cell line derived fr BALB/c ms mammary tumor
Blocking :
1% BSA in PBS (pH7.6), 22°C, 1Hr
Fixation :
95% methnol
Permeabilization /Antigen retrieval :
0.2% Triton X-100 in PBS (pH7.6), 15min, 4°C
Primary Antibody :
1:100, 4°C, 16Hr
Lane Description :
Red: GTX100539 (Alxa Fluor 568)
Blue: DAPI
Date :
Anonymous submitted on 10-Aug-2021
Application Tested :
WB
Sample Species :
Rat
Figure Note :
The data was peer reviewed and published in the journal Biomolecules in 2019.
PMID: 31614639
Date :
Anonymous submitted on 10-Aug-2021
Application Tested :
WB
Sample Species :
Hu
Figure Note :
The data was peer reviewed and published in the journal Cell Death Dis in 2014.
PMID: 25429617
Date :
Anonymous submitted on 10-Aug-2021
Application Tested :
WB
Sample Species :
Rat
Figure Note :
The data was peer reviewed and published in the journal Sci Rep in 2016.
PMID: 27659793
Date :
Anonymous submitted on 18-Mar-2021
Application Tested :
IHC-P
Sample Species :
Hu
Figure Note :
The data was peer reviewed and published in the journal Sci Rep in 2017.
PMID: 28112237
Date :
Anonymous submitted on 18-Mar-2021
Application Tested :
WB
Sample Species :
Ms
Figure Note :
The data was peer reviewed and published in the journal Toxins (Basel) in 2013.
PMID: 24335753
Date :
Anonymous submitted on 18-Mar-2021
Application Tested :
WB
Sample Species :
Hu
Figure Note :
The data was peer reviewed and published in the journal Oncotarget in 2017.
PMID: 27458161
Date :
Anonymous submitted on 21-Oct-2020
Application Tested :
IHC-P
Sample Species :
Hu
Sample :
normal colon tissue
Fixation :
Citrate buffer, 0.3°C
Primary Antibody :
1:500, 4°C, 16Hr
Date :
Anonymous submitted on 01-Oct-2019
Application Tested :
WB
Sample Species :
Golden Syrian Hamster
Sample :
BHK21 cells
Amount used :
5 μg
Blocking :
2.5% non-fat milk, 25°C, 0.5Hr
Primary Antibody :
1:2000, 4°C, 16Hr
Date :
Anonymous submitted on 05-Jul-2018
Application Tested :
ICC/IF
Sample Species :
Hu
Sample :
PANC-1 cells
Blocking :
PBS with 2% FBS and 0.4% Triton X-100, 25°C, 0.17Hr
Fixation :
4% paraformaldehyde
Permeabilization /Antigen retrieval :
PBS with 2% FBS and 0.4% Triton X-100
Primary Antibody :
1:100, 4°C, 16Hr
Date :
Anonymous submitted on 23-May-2016
Application Tested :
WB
Sample Species :
Hu
Sample :
HeLa cells
Amount used :
20μg
Blocking :
5% milk in TBST, 25°C, 1Hr
Primary Antibody :
1:1000, 25°C, 2Hr
Date :
Anonymous submitted on 30-Jan-2012
Application Tested :
WB
Sample Species :
Hu
Sample :
A549 cells,SiHa cells,HeLa cells
Amount used :
10μg
Blocking :
5% milk, 25°C, 1Hr
Primary Antibody :
1:1000, 4°C, 16Hr
