Application Note
IHC-P: Use at a dilution of 1/200 for 10 min at RT. No special pretreatment is required for the immunohistochemical staining of formalin/paraffin tissues. WB: Use at an assay dependent dilution. Predicted molecular weight: 31 kDa. Optimal dilutions/concentrations should be determined by the end user.
Calculated MW
Positive Control
Tonsil, lymph node, or small intestine
Form
Liquid
Buffer
PBS, 1% BSA
Preservative
0.09% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. Store at 4ºC. DO NOT FREEZE.
Concentration
0.2 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Recombinant human PCNA protein
Purification
Immunogen affinity purified
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
Synonyms
proliferating cell nuclear antigen , ATLD2
Cellular Localization
Nucleus
Background
The protein encoded by this gene is found in the nucleus and is a cofactor of DNA polymerase delta. The encoded protein acts as a homotrimer and helps increase the processivity of leading strand synthesis during DNA replication. In response to DNA damage, this protein is ubiquitinated and is involved in the RAD6-dependent DNA repair pathway. Two transcript variants encoding the same protein have been found for this gene. Pseudogenes of this gene have been described on chromosome 4 and on the X chromosome. [provided by RefSeq, Jul 2008]
Database
Research Area