GeneTex
United States (US)

PCNA antibody

PCNA-antibody_IHC-P_GTX15497-1_18121411_413.jpg

Cat No. GTX15497

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, IHC-P
Reactivity Human
APPLICATION

Application Note

IHC-P: Use at a dilution of 1/200 for 10 min at RT. No special pretreatment is required for the immunohistochemical staining of formalin/paraffin tissues. WB: Use at an assay dependent dilution. Predicted molecular weight: 31 kDa. Optimal dilutions/concentrations should be determined by the end user.

Calculated MW

29 kDa. ( Note )

Positive Control

Tonsil, lymph node, or small intestine
PROPERTIES

Form

Liquid

Buffer

Phosphate-buffered saline, pH 7.4 containing 1% BSA and 0.09% sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. Store at 4ºC. DO NOT FREEZE.

Concentration

0.2 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant human PCNA protein

Purification

Immunogen affinity purified

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Proliferating Cell Nuclear Antigen,Atld2,Pcna

Cellular Localization

Nuclear

Background

PCNA is a marker for cells in early G1 phase and S phase of the cell cycle. It is found in the nucleus and is a cofactor of DNA polymerase delta. It acts as a homotrimer and helps increase the processivity of leading strand synthesis during DNA replication. In response to DNA damage, PCNA is ubiquitinated and is involved in the RAD6-dependent DNA repair pathway. Two transcript variants encoding the same protein have been found for PCNA. Pseudogenes of this gene have been described on chromosome 4 and on the X chromosome.

Database

Research Area

DATA IMAGES
PCNA-antibody_IHC-P_GTX15497-1_18121411_413.jpg

GTX15497 IHC-P Image

Human Tonsil stained with anti-PCNA antibody (GTX15497)

Package List Price ($)
$ 289