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PCOLCE antibody [N1C3]

Anti-PCOLCE antibody [N1C3] used in Western Blot (WB). GTX104154
Anti-PCOLCE antibody [N1C3] used in IHC (Paraffin sections) (IHC-P). GTX104154
Anti-PCOLCE antibody [N1C3] used in Western Blot (WB). GTX104154

Cat No. GTX104154

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, IHC-P

Reactivity

Human
Package
100 μl ($329),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

48 kDa. ( Note )

Positive Control

HeLa

Predict Reactivity

Bovine(>80% identity)

PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.69 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human PCOLCE. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

procollagen C-endopeptidase enhancer , PCPE , PCPE-1 , PCPE1

Cellular Localization

Secreted

Background

Fibrillar collagen types I-III are synthesized as precursor molecules known as procollagens. These precursors contain amino- and carboxyl-terminal peptide extensions known as N- and C-propeptides, respectively, which are cleaved, upon secretion of procollagen from the cell, to yield the mature triple helical, highly structured fibrils. This gene encodes a glycoprotein which binds and drives the enzymatic cleavage of type I procollagen and heightens C-proteinase activity. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-PCOLCE antibody [N1C3] used in Western Blot (WB). GTX104154

GTX104154 WB Image

Sample (30 ug of whole cell lysate)
A: Hela
10% SDS PAGE
GTX104154 diluted at 1:1000

Anti-PCOLCE antibody [N1C3] used in IHC (Paraffin sections) (IHC-P). GTX104154

GTX104154 IHC-P Image

Immunohistochemical analysis of paraffin-embedded SAS xenograft, using PCPE-1(GTX104154) antibody at 1:100 dilution.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-PCOLCE antibody [N1C3] used in Western Blot (WB). GTX104154

GTX104154 WB Image

Whole cell extract (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with PCOLCE antibody [N1C3] (GTX104154) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

There are currently no references for PCOLCE antibody [N1C3] (GTX104154). Be the first to share your publications with this product.

REVIEW

There are currently no reviews for PCOLCE antibody [N1C3] (GTX104154). Be the first to share your experience with this product.
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf