GeneTex
United States (US)

PCOLCE antibody

Cat No. GTX11577

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB
Reactivity Human, Mouse, Pig
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB Assay dependent
Not tested in other applications.

Calculated MW

48 kDa. ( Note )

Specificity/Sensitivity

Recognizes both full-sized and cleaved PCOLE-1. Does not react with PCOLE-2.
PROPERTIES

Form

Liquid

Buffer

0.01M PBS PH 7.4, 0.05% Sodium Azide, 50% Glycerol

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. Store at 4ºC. DO NOT FREEZE.

Antigen Species

Human

Immunogen

Synthetic peptide corresponding to the end of CUB2 and the beginning of the “linker” region of PCOLCE (Human).

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Procollagen C-Endopeptidase Enhancer,Pcpe,Pcpe-1,Pcpe1,Pcolce

Background

BMP1, also known as Bone Morphogenetic Protein 1, Procollagen C-endopeptidase, Procollagen C-peptidase, or Procollagen C-proteinase, is an extracellular zinc endopeptidase of the astacin family. PCOLE1 was described as an accessory protein that enhances the activity of BMP1. Another BMP1 enhancer protein, PCOLE2, was described in a subtractive library of eye tissue, and little is known about its function. PCOLE1 binds to Procollagen and assists BMP1, increasing the Type-I Procollagen C-Terminal processing activity 10-fold, although it does not directly activate the enzyme. BMP1 is produced in a number of different forms, with different C-terminal regions. PCOLE1 and 2 may be isoform- or tissuespecific enhancers, giving a degree of regulation to the activity of BMP1 in different circumstances. PCOLE1 is produced in tissues rich in Type-I collagen, such as skin, kidney and tendon. It is secreted into culture media by a wide range of cell types.

Database

Package List Price ($)
$ 289